Michael J Dark

Michael J Dark,

Executive Director, Veterinary Diagnostic Laboratories

Department: VM-ADMINISTRATION
Business Phone: (352) 294-4138
Business Email: darkmich@ufl.edu

Accomplishments

Barbara Jean Thompson Award for Outstanding Service
2018 · Davis / Thompson Foundation
Member, Board of Directors
2017-2025 · Davis / Thompson Foundation

Board Certifications

  • Diplomate
    American College of Veterinary Pathologists

Research Profile

Veterinary Pathology, including studying the pathology of a variety of conditions in animals in an attempt to better model human disease.

Discovery and diagnosis of diseases in animals

Bacterial genomics and vector-borne diseases

Bacterial genome sequencing and bioinformatics

Rickettsial diseases such as Anaplasma marginale and other model systems

Disease research, including how to better use archived samples for genetic research

Examining alternative fixatives for DNA research

Determining the maximum useful sample age for DNA recovery from diagnostic tissue samples.

Animals as model systems for disease

Pathology of a variety of conditions in animals in an attempt to better model human disease.

Open Researcher and Contributor ID (ORCID)

0000-0002-6547-1298

Publications

2021
Canine osteosarcoma checkpoint expression correlates with metastasis and T-cell infiltrate.
Veterinary immunology and immunopathology. 232 [DOI] 10.1016/j.vetimm.2020.110169. [PMID] 33387703.
2021
Evaluation of ponatinib in vitro effect in three canine mast cell tumor cell lines expressing FGFR-1, PDGFR-α, and VEGFR-2.
Veterinary journal (London, England : 1997). 269 [DOI] 10.1016/j.tvjl.2021.105621. [PMID] 33593493.
2021
International Guidelines for Veterinary Tumor Pathology: A Call to Action.
Veterinary pathology. [DOI] 10.1177/03009858211013712. [PMID] 34282984.
2021
Retrospective study of gross and histopathologic lesions associated with closed chest cardiopulmonary resuscitation in dogs.
The Journal of small animal practice. 62(9):750-755 [DOI] 10.1111/jsap.13349. [PMID] 33987841.
2020
Feasibility and efficacy of ultrasonographic and laparoscopic guidance for microwave ablation of clinically normal canine ovaries.
American journal of veterinary research. 81(9):747-754 [DOI] 10.2460/ajvr.81.9.747. [PMID] 33112170.
2020
Primary Hyperparathyroidism in a Quarter Horse Mare Associated With a Chief Cell Adenoma.
Journal of equine veterinary science. 95 [DOI] 10.1016/j.jevs.2020.103302. [PMID] 33276927.
2020
SDHB and SDHA Immunohistochemistry in Canine Pheochromocytomas.
Animals : an open access journal from MDPI. 10(9) [DOI] 10.3390/ani10091683. [PMID] 32957698.
2020
What is your diagnosis? Cutaneous mass from a Shar-Pei Dog.
Veterinary clinical pathology. 49(2):365-366 [DOI] 10.1111/vcp.12854. [PMID] 32347557.
2019
Complete Urogenital and Anorectal Duplication in a Dog.
Case reports in veterinary medicine. 2019 [DOI] 10.1155/2019/3696978. [PMID] 30941228.
2019
Fine-needle aspirate of a large mass in the ventral peritoneal space of a dog.
Veterinary clinical pathology. 48(2):355-357 [DOI] 10.1111/vcp.12716. [PMID] 30938837.
2019
Immunogenicity and Efficacy of a Novel Multi-Antigenic Peptide Vaccine Based on Cross-Reactivity between Feline and Human Immunodeficiency Viruses.
Viruses. 11(2) [DOI] 10.3390/v11020136. [PMID] 30717485.
2019
Laparoscopic access to the liver and application of laparoscopic microwave ablation in 2 dogs with liver neoplasia.
Veterinary surgery : VS. 48(S1):O91-O98 [DOI] 10.1111/vsu.13153. [PMID] 30666685.
2019
Pathology in Practice.
Journal of the American Veterinary Medical Association. 255(3):309-312 [DOI] 10.2460/javma.255.3.309. [PMID] 31298650.
2019
Understanding the Efficiency of Splenic Hemangiosarcoma Diagnosis Using Monte Carlo Simulations.
Veterinary pathology. 56(6):856-859 [DOI] 10.1177/0300985819868732. [PMID] 31422751.
2018
Gene Therapy for Osteoarthritis: Pharmacokinetics of Intra-Articular Self-Complementary Adeno-Associated Virus Interleukin-1 Receptor Antagonist Delivery in an Equine Model.
Human gene therapy. Clinical development. 29(2):90-100 [DOI] 10.1089/humc.2017.142. [PMID] 29869540.
2018
Histologic characterization of eosinophilic encephalitis in horses in Florida.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 30(3):442-446 [DOI] 10.1177/1040638718763877. [PMID] 29528809.
2018
Self-Complementary Adeno-Associated Virus-Mediated Interleukin-1 Receptor Antagonist Gene Delivery for the Treatment of Osteoarthritis: Test of Efficacy in an Equine Model.
Human gene therapy. Clinical development. 29(2):101-112 [DOI] 10.1089/humc.2017.143. [PMID] 29869535.
2018
Viral Enrichment Methods Affect the Detection but Not Sequence Variation of West Nile Virus in Equine Brain Tissue.
Frontiers in veterinary science. 5 [DOI] 10.3389/fvets.2018.00318. [PMID] 30619900.
2017
A retrospective study of the prevalence of calcium oxalate crystals in veterinary Aspergillus cases.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 29(1):51-58 [DOI] 10.1177/1040638716672254. [PMID] 27852812.
2017
Cutaneous iridophoroma in a Green iguana ‬‬‬‬‬‬‬‬‬‬‬‬‬(Iguana iguana)‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬.
Veterinary clinical pathology. 46(4):625-628 [DOI] 10.1111/vcp.12536. [PMID] 28815678.
2017
Determination of metabolic resistance mechanisms in pyrethroid-resistant and fipronil-tolerant brown dog ticks.
Medical and veterinary entomology. 31(3):243-251 [DOI] 10.1111/mve.12240. [PMID] 28639697.
2017
Microwave ablation for treatment of hepatic neoplasia in five dogs.
Journal of the American Veterinary Medical Association. 250(1):79-85 [DOI] 10.2460/javma.250.1.79. [PMID] 28001112.
2016
Chordomas at High Prevalence in the Captive Population of the Endangered Perdido Key Beach Mouse (Peromyscus polionotus trissyllepsis).
Veterinary pathology. 53(1):163-9 [DOI] 10.1177/0300985815575051. [PMID] 25791038.
2016
Determining the utility of veterinary tissue archives for retrospective DNA analysis.
PeerJ. 4 [DOI] 10.7717/peerj.1996. [PMID] 27168995.
2016
Superinfection Exclusion of the Ruminant Pathogen Anaplasma marginale in Its Tick Vector Is Dependent on the Time between Exposures to the Strains.
Applied and environmental microbiology. 82(11):3217-3224 [DOI] 10.1128/AEM.00190-16. [PMID] 26994084.
2014
Comparison of histomorphology and DNA preservation produced by fixatives in the veterinary diagnostic laboratory setting.
PeerJ. 2 [DOI] 10.7717/peerj.377. [PMID] 24860702.
2014
Efficacy of slow-release tags impregnated with aggregation-attachment pheromone and deltamethrin for control of Amblyomma variegatum on St. Kitts, West Indies.
Parasites & vectors. 7 [DOI] 10.1186/1756-3305-7-182. [PMID] 24731252.
2014
Evaluation of the effect of formalin fixation on skin specimens in dogs and cats.
PeerJ. 2 [DOI] 10.7717/peerj.307. [PMID] 24688882.
2013
Gastrointestinal leiomyosarcoma in a pygmy sperm whale (Kogia breviceps).
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians. 44(3):744-8 [PMID] 24063105.
View on: PubMed
2013
scAAV-mediated gene transfer of interleukin-1-receptor antagonist to synovium and articular cartilage in large mammalian joints.
Gene therapy. 20(6):670-7 [DOI] 10.1038/gt.2012.81. [PMID] 23151520.
2013
T Cell Rich, B Cell Lymphoma in the Small Colon of a Yearling Horse
Equine Veterinary Education. 25(2):74-78 [DOI] 10.1111/j.2042-3292.2012.00398.x.
2013
Whole-genome sequencing in bacteriology: state of the art.
Infection and drug resistance. 6:115-23 [DOI] 10.2147/IDR.S35710. [PMID] 24143115.
2012
Comparative genomics and transcriptomics of trait-gene association.
BMC genomics. 13 [DOI] 10.1186/1471-2164-13-669. [PMID] 23181781.
2012
Determining the repertoire of immunodominant proteins via whole-genome amplification of intracellular pathogens.
PloS one. 7(4) [DOI] 10.1371/journal.pone.0036456. [PMID] 22558468.
2012
Ocular and Olfactory Forebrain Abnormalities Within a Neonatal Alpaca (Vicugna Pacos)
Journal of Veterinary Medical Science. 74(4):945-947 [DOI] 10.1292/jvms.11-0558. [PMID] 24280395.
2012
Ocular and olfactory forebrain abnormalities within a neonatal alpaca (Vicugna pacos).
The Journal of veterinary medical science. 74(7):945-7 [PMID] 22446393.
View on: PubMed
2011
Multistrain genome analysis identifies candidate vaccine antigens of Anaplasma marginale.
Vaccine. 29(31):4923-32 [DOI] 10.1016/j.vaccine.2011.04.131. [PMID] 21596083.
2009
Conservation in the face of diversity: multistrain analysis of an intracellular bacterium.
BMC genomics. 10 [DOI] 10.1186/1471-2164-10-16. [PMID] 19134224.
2008
Evidence for persistent Bovine viral diarrhea virus infection in a captive mountain goat (Oreamnos americanus).
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 20(6):752-9 [PMID] 18987224.
View on: PubMed
2008
Granulocytopenia associated with thymoma in a domestic shorthaired cat.
Journal of the American Animal Hospital Association. 44(4):210-7 [PMID] 18593858.
View on: PubMed
2008
Safety and efficacy of intramuscular propofol administration in rats.
Veterinary anaesthesia and analgesia. 35(6):495-500 [DOI] 10.1111/j.1467-2995.2008.00418.x. [PMID] 18980630.
2008
Superinfection as a driver of genomic diversification in antigenically variant pathogens.
Proceedings of the National Academy of Sciences of the United States of America. 105(6):2123-7 [DOI] 10.1073/pnas.0710333105. [PMID] 18252822.
2007
Immunogenicity of Anaplasma marginale type IV secretion system proteins in a protective outer membrane vaccine.
Infection and immunity. 75(5):2333-42 [PMID] 17339347.
View on: PubMed
2006
Differential expression and sequence conservation of the Anaplasma marginale msp2 gene superfamily outer membrane proteins.
Infection and immunity. 74(6):3471-9 [PMID] 16714578.
View on: PubMed
2005
Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins.
Proceedings of the National Academy of Sciences of the United States of America. 102(3):844-9 [PMID] 15618402.
View on: PubMed
2002
Autosomal recessive severe combined immunodeficiency of Jack Russell terriers.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 14(3):194-204 [PMID] 12033674.
View on: PubMed
2001
SCID in Jack Russell terriers: a new animal model of DNA-PKcs deficiency.
Journal of immunology (Baltimore, Md. : 1950). 167(4):2142-50 [PMID] 11489998.
View on: PubMed

Education

Diplomate
2011 · American College of Veterinary Pathologists
PhD, Veterinary Microbiology & Pathology
2008 · Washington State University
Doctor of Veterinary Medicine
2001 · Michigan State University

Teaching Profile

Courses Taught
2014-2015,2017-2021,2020-2021
VEM5115 Veterinary Histology
2011-2013,2015-2019,2017-2021
VEM5851 Senior Clk App Pathol
2010-2021
VEM5751 Core Clk Appl Pathol
2012-2013,2015-2020,2019-2021
VEM5571 Emerging Diseases
2011-2021
VEM5165 Large Anim Pathology
2011-2020
VEM5162 Vet Pathology 2
2011-2016,2018-2019
VEM5164 Small Anim Pathology
2018
VME7980 Research for Doctoral Dissertation

Contact Details

Phones:
Business:
(352) 294-4138
Emails:
Business:
darkmich@ufl.edu