Michael J Dark,
Director & Clinical Associate Professor
Department:
Department of Comparative, Diagnostic & Population Medicine
Business Phone:
(352) 294-4138
Business Email:
darkmich@ufl.edu
Accomplishments
Barbara Jean Thompson Award for Outstanding Service
2018
·
Davis / Thompson Foundation
Member, Board of Directors
2017-2025
·
Davis / Thompson Foundation
Teaching Profile
Courses Taught
2014-2015,2017-2021,2020-2023
VEM5115 Veterinary Histology
2011-2013,2015-2019,2017-2025,2023-2025
VEM5851 Adv. Anatomic Pathology Clerkship
2010-2021,2023-2025
VEM5751 Anatomic Path Core Clerkship
2012-2013,2015-2020,2019-2025
VEM5571 Emerging Diseases
2011-2025
VEM5165 Large Anim Pathology
2011-2020,2025
VEM5162 Vet Pathology 2
2011-2016,2018-2019
VEM5164 Small Anim Pathology
2018
VME7980 Research for Doctoral Dissertation
Board Certifications
-
DiplomateAmerican College of Veterinary Pathologists
Research Profile
Veterinary Pathology, including studying the pathology of a variety of conditions in animals in an attempt to better model human disease.
Discovery and diagnosis of diseases in animals
Bacterial genomics and vector-borne diseases
Bacterial genome sequencing and bioinformatics
Rickettsial diseases such as Anaplasma marginale and other model systems
Disease research, including how to better use archived samples for genetic research
Examining alternative fixatives for DNA research
Determining the maximum useful sample age for DNA recovery from diagnostic tissue samples.
Animals as model systems for disease
Pathology of a variety of conditions in animals in an attempt to better model human disease.
Publications
Academic Articles
2025
Nuclear pleomorphism in canine cutaneous mast cell tumors: Comparison of reproducibility and prognostic relevance between estimates, manual morphometry, and algorithmic morphometry
Veterinary Pathology.
62(2):161-177
[DOI] 10.1177/03009858241295399.
[PMID] 39560067.
2024
Automated Nuclear Morphometry: A Deep Learning Approach for Prognostication in Canine Pulmonary Carcinoma to Enhance Reproducibility
Veterinary Sciences.
11(6)
[DOI] 10.3390/vetsci11060278.
[PMID] 38922025.
2024
Information mismatch in PHH3-assisted mitosis annotation leads to interpretation shifts in H&E slide analysis.
Scientific reports.
14(1)
[DOI] 10.1038/s41598-024-77244-6.
[PMID] 39487193.
2024
Keeping Pathologists in the Loop and an Adaptive F1-Score Threshold Method for Mitosis Detection in Canine Perivascular Wall Tumours
Cancers.
16(3)
[DOI] 10.3390/cancers16030644.
[PMID] 38339394.
2024
Overview of the Veterinary Cancer Guidelines and Protocols group’s “Developing, reporting and validating histologic tumor grading systems”
Veterinary Pathology.
61(1):157-159
[DOI] 10.1177/03009858231209409.
2024
Telangiectatic osteosarcoma in four dogs: Cytologic, histopathologic, cytochemical, and immunohistochemical findings.
Veterinary clinical pathology.
53(1):85-92
[DOI] 10.1111/vcp.13338.
[PMID] 38418390.
2023
Chronic intermittent dyschezia in a 10-year-old spayed female domestic shorthair cat.
Journal of the American Veterinary Medical Association.
261(6):1-4
[DOI] 10.2460/javma.22.11.0502.
[PMID] 36626289.
2023
Detection of Necrosis in Digitised Whole-Slide Images for Better Grading of Canine Soft-Tissue Sarcomas Using Machine-Learning
Veterinary Sciences.
10(1)
[DOI] 10.3390/vetsci10010045.
[PMID] 36669046.
2023
Mitosis domain generalization in histopathology images – The MIDOG challenge.
Medical image analysis.
84
[DOI] 10.1016/j.media.2022.102699.
[PMID] 36463832.
2023
Multiple biliary duct hamartomas in a cat resulting in a hepatic mass: A case report.
Veterinary medicine and science.
9(4):1441-1445
[DOI] 10.1002/vms3.1175.
[PMID] 37386741.
2022
Deep learning for necrosis detection using canine perivascular wall tumour whole slide images.
Scientific reports.
12(1)
[DOI] 10.1038/s41598-022-13928-1.
[PMID] 35739267.
2022
Magnetic resonance imaging of the normal equine pituitary gland
Veterinary Radiology & Ultrasound.
63(4):450-455
[DOI] 10.1111/vru.13072.
2022
RNA in situ hybridisation as a molecular diagnostic technique targeting IBA-1 and CD204 in canine histiocytic sarcoma.
Veterinary medicine and science.
8(4):1400-1408
[DOI] 10.1002/vms3.795.
[PMID] 35339118.
2021
Canine osteosarcoma checkpoint expression correlates with metastasis and T-cell infiltrate.
Veterinary immunology and immunopathology.
232
[DOI] 10.1016/j.vetimm.2020.110169.
[PMID] 33387703.
2021
Evaluation of ponatinib in vitro effect in three canine mast cell tumor cell lines expressing FGFR-1, PDGFR-α, and VEGFR-2
The Veterinary Journal.
269
[DOI] 10.1016/j.tvjl.2021.105621.
2021
International Guidelines for Veterinary Tumor Pathology: A Call to Action.
Veterinary pathology.
58(5):766-794
[DOI] 10.1177/03009858211013712.
[PMID] 34282984.
2021
Retrospective study of gross and histopathologic lesions associated with closed chest cardiopulmonary resuscitation in dogs.
The Journal of small animal practice.
62(9):750-755
[DOI] 10.1111/jsap.13349.
[PMID] 33987841.
2020
Feasibility and efficacy of ultrasonographic and laparoscopic guidance for microwave ablation of clinically normal canine ovaries.
American journal of veterinary research.
81(9):747-754
[DOI] 10.2460/ajvr.81.9.747.
[PMID] 33112170.
2020
Primary Hyperparathyroidism in a Quarter Horse Mare Associated With a Chief Cell Adenoma
Journal of Equine Veterinary Science.
95
[DOI] 10.1016/j.jevs.2020.103302.
2020
SDHB and SDHA Immunohistochemistry in Canine Pheochromocytomas
Animals.
10(9)
[DOI] 10.3390/ani10091683.
[PMID] 32957698.
2020
What is your diagnosis? Cutaneous mass from a Shar‐Pei Dog
Veterinary Clinical Pathology.
49(2):365-366
[DOI] 10.1111/vcp.12854.
2019
Complete Urogenital and Anorectal Duplication in a Dog.
Case reports in veterinary medicine.
2019
[DOI] 10.1155/2019/3696978.
[PMID] 30941228.
2019
Fine-needle aspirate of a large mass in the ventral peritoneal space of a dog.
Veterinary clinical pathology.
48(2):355-357
[DOI] 10.1111/vcp.12716.
[PMID] 30938837.
2019
Immunogenicity and Efficacy of a Novel Multi-Antigenic Peptide Vaccine Based on Cross-Reactivity between Feline and Human Immunodeficiency Viruses.
Viruses.
11(2)
[DOI] 10.3390/v11020136.
[PMID] 30717485.
2019
Laparoscopic access to the liver and application of laparoscopic microwave ablation in 2 dogs with liver neoplasia.
Veterinary surgery : VS.
48(S1):O91-O98
[DOI] 10.1111/vsu.13153.
[PMID] 30666685.
2019
Pathology in Practice.
Journal of the American Veterinary Medical Association.
255(3):309-312
[DOI] 10.2460/javma.255.3.309.
[PMID] 31298650.
2019
Understanding the Efficiency of Splenic Hemangiosarcoma Diagnosis Using Monte Carlo Simulations.
Veterinary pathology.
56(6):856-859
[DOI] 10.1177/0300985819868732.
[PMID] 31422751.
2018
Gene Therapy for Osteoarthritis: Pharmacokinetics of Intra-Articular Self-Complementary Adeno-Associated Virus Interleukin-1 Receptor Antagonist Delivery in an Equine Model.
Human gene therapy. Clinical development.
29(2):90-100
[DOI] 10.1089/humc.2017.142.
[PMID] 29869540.
2018
Histologic characterization of eosinophilic encephalitis in horses in Florida.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.
30(3):442-446
[DOI] 10.1177/1040638718763877.
[PMID] 29528809.
2018
Self-Complementary Adeno-Associated Virus-Mediated Interleukin-1 Receptor Antagonist Gene Delivery for the Treatment of Osteoarthritis: Test of Efficacy in an Equine Model.
Human gene therapy. Clinical development.
29(2):101-112
[DOI] 10.1089/humc.2017.143.
[PMID] 29869535.
2018
Viral Enrichment Methods Affect the Detection but Not Sequence Variation of West Nile Virus in Equine Brain Tissue.
Frontiers in veterinary science.
5
[DOI] 10.3389/fvets.2018.00318.
[PMID] 30619900.
2017
A retrospective study of the prevalence of calcium oxalate crystals in veterinary Aspergillus cases.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.
29(1):51-58
[DOI] 10.1177/1040638716672254.
[PMID] 27852812.
2017
Cutaneous iridophoroma in a Green iguana (Iguana iguana).
Veterinary clinical pathology.
46(4):625-628
[DOI] 10.1111/vcp.12536.
[PMID] 28815678.
2017
Determination of metabolic resistance mechanisms in pyrethroid-resistant and fipronil-tolerant brown dog ticks.
Medical and veterinary entomology.
31(3):243-251
[DOI] 10.1111/mve.12240.
[PMID] 28639697.
2017
Microwave ablation for treatment of hepatic neoplasia in five dogs.
Journal of the American Veterinary Medical Association.
250(1):79-85
[DOI] 10.2460/javma.250.1.79.
[PMID] 28001112.
2016
Chordomas at High Prevalence in the Captive Population of the Endangered Perdido Key Beach Mouse (Peromyscus polionotus trissyllepsis).
Veterinary pathology.
53(1):163-9
[DOI] 10.1177/0300985815575051.
[PMID] 25791038.
2016
Determining the utility of veterinary tissue archives for retrospective DNA analysis.
PeerJ.
4
[DOI] 10.7717/peerj.1996.
[PMID] 27168995.
2016
Superinfection Exclusion of the Ruminant Pathogen Anaplasma marginale in Its Tick Vector Is Dependent on the Time between Exposures to the Strains.
Applied and environmental microbiology.
82(11):3217-3224
[DOI] 10.1128/AEM.00190-16.
[PMID] 26994084.
2014
Comparison of histomorphology and DNA preservation produced by fixatives in the veterinary diagnostic laboratory setting.
PeerJ.
2
[DOI] 10.7717/peerj.377.
[PMID] 24860702.
2014
Efficacy of slow-release tags impregnated with aggregation-attachment pheromone and deltamethrin for control of Amblyomma variegatum on St. Kitts, West Indies.
Parasites & vectors.
7
[DOI] 10.1186/1756-3305-7-182.
[PMID] 24731252.
2014
Evaluation of the effect of formalin fixation on skin specimens in dogs and cats.
PeerJ.
2
[DOI] 10.7717/peerj.307.
[PMID] 24688882.
2013
Anorectal anatomy and imaging techniques.
Gastroenterology clinics of North America.
42(4):701-12
[DOI] 10.1016/j.gtc.2013.09.008.
[PMID] 24280395.
2013
Gastrointestinal leiomyosarcoma in a pygmy sperm whale (Kogia breviceps).
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.
44(3):744-8
[PMID] 24063105.
2013
scAAV-mediated gene transfer of interleukin-1-receptor antagonist to synovium and articular cartilage in large mammalian joints.
Gene therapy.
20(6):670-7
[DOI] 10.1038/gt.2012.81.
[PMID] 23151520.
2013
T Cell Rich, B Cell Lymphoma in the Small Colon of a Yearling Horse
Equine Veterinary Education.
25(2):74-78
[DOI] 10.1111/j.2042-3292.2012.00398.x.
2013
Whole-genome sequencing in bacteriology: state of the art.
Infection and drug resistance.
6:115-23
[DOI] 10.2147/IDR.S35710.
[PMID] 24143115.
2012
Comparative genomics and transcriptomics of trait-gene association.
BMC genomics.
13
[DOI] 10.1186/1471-2164-13-669.
[PMID] 23181781.
2012
Determining the repertoire of immunodominant proteins via whole-genome amplification of intracellular pathogens.
PloS one.
7(4)
[DOI] 10.1371/journal.pone.0036456.
[PMID] 22558468.
2012
Ocular and olfactory forebrain abnormalities within a neonatal alpaca (Vicugna pacos).
The Journal of veterinary medical science.
74(7):945-7
[PMID] 22446393.
2011
Multistrain genome analysis identifies candidate vaccine antigens of Anaplasma marginale.
Vaccine.
29(31):4923-32
[DOI] 10.1016/j.vaccine.2011.04.131.
[PMID] 21596083.
2009
Conservation in the face of diversity: multistrain analysis of an intracellular bacterium.
BMC genomics.
10
[DOI] 10.1186/1471-2164-10-16.
[PMID] 19134224.
2008
Evidence for persistent Bovine viral diarrhea virus infection in a captive mountain goat (Oreamnos americanus).
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.
20(6):752-9
[PMID] 18987224.
2008
Granulocytopenia associated with thymoma in a domestic shorthaired cat.
Journal of the American Animal Hospital Association.
44(4):210-7
[PMID] 18593858.
2008
Safety and efficacy of intramuscular propofol administration in rats.
Veterinary anaesthesia and analgesia.
35(6):495-500
[DOI] 10.1111/j.1467-2995.2008.00418.x.
[PMID] 18980630.
2008
Superinfection as a driver of genomic diversification in antigenically variant pathogens.
Proceedings of the National Academy of Sciences of the United States of America.
105(6):2123-7
[DOI] 10.1073/pnas.0710333105.
[PMID] 18252822.
2007
Immunogenicity of Anaplasma marginale type IV secretion system proteins in a protective outer membrane vaccine.
Infection and immunity.
75(5):2333-42
[PMID] 17339347.
2006
Differential expression and sequence conservation of the Anaplasma marginale msp2 gene superfamily outer membrane proteins.
Infection and immunity.
74(6):3471-9
[PMID] 16714578.
2005
Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins.
Proceedings of the National Academy of Sciences of the United States of America.
102(3):844-9
[PMID] 15618402.
2002
Autosomal recessive severe combined immunodeficiency of Jack Russell terriers.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.
14(3):194-204
[PMID] 12033674.
2001
SCID in Jack Russell terriers: a new animal model of DNA-PKcs deficiency.
Journal of immunology (Baltimore, Md. : 1950).
167(4):2142-50
[PMID] 11489998.
Education
Diplomate
2011
·
American College of Veterinary Pathologists
PhD, Veterinary Microbiology & Pathology
2008
·
Washington State University
Doctor of Veterinary Medicine
2001
·
Michigan State University
Contact Details
Phones:
- Business:
- (352) 294-4138
Emails:
- Business:
- darkmich@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 100123
GAINESVILLE FL 32610 - Business Street:
-
COLLEGE OF VETERINARY MEDICINE
VS-2B
VCS
GAINESVILLE FL 32610