Christopher Vulpe

Christopher Vulpe, MD, PhD

Professor

Department: Department of Physiological Sciences
Business Phone: (650) 291-5679
Business Email: cvulpe@ufl.edu

Research Profile

Ecotoxicology

We are developing novel approaches for identifying and understanding the toxicity of chemicals and nanomaterials in aquatic ecosystems. We are focused on the aquatic indicator species Daphnia magna (a crustacean) as well as representative fish species such as Rainbow Trout. We haveMost recently, we are focused on developing a mechanistic understanding the effects of silver nanowires in these species. We are also developing CRISPR based approaches to assess the function of toxicant related genes.

Genetics of Iron Homeostasis

Genetic variants influence iron homeostasis in mammals. My group has utilized genome wide approaches to identify genetic factors that influence iron status in mammals. Recently, we performed an association analysis of over 100 inbred strains of mice to identify genetic determinants of iron status in collaboration with Jake Lusis and Eleazar Eskin at UCLA. Ongoing work is to determine the functional role of candidate genes identified in these studies.

Mammalian ferroxidases – Hephaestin

Intestinal iron absorption is remarkably balanced to provide adequate iron to meet the body’s iron needs while preventing toxic excess. In a collaboration with Greg Anderson from QIMR, Brisbane Australia, Hephaestin (Hp) was identified to be a membrane-bound copper containing ferrroxidase required for export of iron from the intestinal enterocyte into the circulation. We have utilized floxed Hephaestin (Heph) to generate both complete and tissue specific knockouts confirming that global and intestinal specific knockouts result in systemic iron deficiency. Furthermore, we have developed mice which lack both Hephaestin and Ceruloplasmin, the circulating ferroxidase, which develop a much more severe phenotype of iron deficiency than either alone and provide compelling evidence for complementary and partially compensatory functions. In collaboration with Huijun Chen, at Nanjing University, we have demonstrated increased iron levels in many regions of the brain in mice lacking the Heph gene. Most recently, we have been analyzing the molecular mechanisms of the hairloss phenotype associated with iron deficiency in Heph KO mice.

Publications

2019 Proceedings of the National Academy of Sciences of the United States of America. Biological Sciences

Grants

Sep 2020 ACTIVE
Development of HITI CRISPR gene therapy for dilated cardiomyopathy in a canine model
SAREPTA THERAPEUTICS · Principal Investigator
Jul 2019 ACTIVE
Role of Bcl11b in CD4+ T cells and innate lymphoid cells
NATL INST OF HLTH NIAID · Project Manager
Jul 2019 ACTIVE
Together: Transforming and Translating Discovery to Improve Health
NATL INST OF HLTH NCATS · Project Manager
Jun 2018 ACTIVE
OR-DRPD-ROD2018: Development of Leukemia-Oncotype Prototype CARS (Cytarabine AML Response Score) for Prediction of Relapse Risk in AML
UF RESEARCH · Project Manager
Sep 2017 ACTIVE
UFHCC Triple Negative Breast Cancer (TNBC) Pilot Project Program
MULTIPLE SPONSORS · Project Manager
Jun 2017 – Apr 2018
CRISPR-CAS9 Genomic Screening COR
US ENVIRONMENTAL PRO AGCY · Principal Investigator
Apr 2017 ACTIVE
OoR CTSI Institutional Matching Support
UF DIV OF SPONSORED RES MATCHING FUNDS · Project Manager
Mar 2017 – Mar 2018
Improved in vitro metabolism in HepG2 cells using CRISPRa Technology
JOHNS HOPKINS UNIV · Principal Investigator
Sep 2016 ACTIVE
Southeastern and Coastal Center for Agricultural Health and Safety (SCC-AHS)
CTRS FOR DIS CNTRL INST OCCUP SFTY HLTH · Project Manager
Sep 2016 ACTIVE
Nanowire Intelligent Re-design and Recycling for Environmental Safety
CONSUMER PRODUCT SAFETY COMM · Principal Investigator
Aug 2016 – Feb 2020
Environmental and Genetic Determinants of Transitional Cell Carcinoma of the Urinary Tract in Scottish Terriers funded by Olive's Way
UF FOU ·
Jul 2016 – Jul 2017
Transform Tox Testing Challenge: Innovating for Metaboli
US ENVIRONMENTAL PRO AGCY · Principal Investigator
Jun 2016 – May 2018
Functional screening to identify mediators of dopaminerg
UF DSR OPPORTUNITY FUND · Principal Investigator
Nov 2015 – Dec 2016
Genomic Differences in response of daphnia magna
INTL COPPER ASSOC · Principal Investigator
Sep 2015 – Jun 2018
High throughput transcriptional profiling of human mammary stem cell denived organoids
LAWRENCE BERKELEY NATL LAB · Principal Investigator
Jun 2015 – May 2018
An Integrated molecular approach to understand
NATL INST OF HLTH NIGMS · Principal Investigator
May 2015 – Apr 2019
Functional Profiling to Identify Mitochondria-cell
NATL INST OF HLTH NIEHS · Principal Investigator
Jan 2015 – May 2020
Investigating Biogeochenical Controls on Metal Toxicity Using Stable Isotopes
COLORADO SCHOOL OF MINES · Principal Investigator

Education

M.D., Medicine
1996 · University of California
Ph.D., Biochemistry, Genetics
1994 · University of California
S.B., Biology
1986 · Massachusetts Institute of Technology

Contact Details

Phones:
Business:
(650) 291-5679
Emails:
Business:
cvulpe@ufl.edu

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