Apichai Tuanyok

Apichai Tuanyok,

Assistant Professor

Department: Department of Infectious Diseases & Immunology
Business Phone: (352) 294-8269
Business Email: tuanyok@ufl.edu

Research Profile

Areas of Interest
  • Burkholderia mallei (Glanders) vaccine development
  • Burkholderia pseudomallei – genotype classification, effective treatment and early diagnosis of Melioidosis and its impact on Southeast Asia
  • Molecular genetics and biology of pathogenic bacteria and their drug resistance

Publications

2021
Use of Recombinant Escherichia coli Strains in Immunofluorescence Assays for Melioidosis Diagnosis.
Pathogens (Basel, Switzerland). 10(5) [DOI] 10.3390/pathogens10050559. [PMID] 34066462.
2020
Genomic loss in environmental and isogenic morphotype isolates of Burkholderia pseudomallei is associated with intracellular survival and plaque-forming efficiency.
PLoS neglected tropical diseases. 14(9) [DOI] 10.1371/journal.pntd.0008590. [PMID] 32991584.
2020
Humoral Immune Responses to Burkholderia pseudomallei Antigens in Captive and Wild Macaques in the Western Part of Java, Indonesia.
Veterinary sciences. 7(4) [DOI] 10.3390/vetsci7040153. [PMID] 33050516.
2020
Investigation of Melioidosis Outbreak in Pig Farms in Southern Thailand.
Veterinary sciences. 7(1) [DOI] 10.3390/vetsci7010009. [PMID] 31947512.
2020
Pathogen to commensal? Longitudinal within-host population dynamics, evolution, and adaptation during a chronic >16-year Burkholderia pseudomallei infection
PLOS Pathogens. 16(3) [DOI] 10.1371/journal.ppat.1008298. [PMID] 32134991.
2019
A novel selective medium for the isolation of Burkholderia mallei from equine specimens.
BMC veterinary research. 15(1) [DOI] 10.1186/s12917-019-1874-0. [PMID] 31064357.
2019
An in situ high-throughput screen identifies inhibitors of intracellular Burkholderia pseudomallei with therapeutic efficacy.
Proceedings of the National Academy of Sciences of the United States of America. 116(37):18597-18606 [DOI] 10.1073/pnas.1906388116. [PMID] 31439817.
2019
Burkholderia pseudomallei acquired ceftazidime resistance due to gene duplication and amplification.
International journal of antimicrobial agents. 53(5):582-588 [DOI] 10.1016/j.ijantimicag.2019.01.003. [PMID] 30639528.
2019
Caprine humoral response to Burkholderia pseudomallei antigens during acute melioidosis from aerosol exposure.
PLoS neglected tropical diseases. 13(2) [DOI] 10.1371/journal.pntd.0006851. [PMID] 30811382.
2019
Successful Treatments and Management of A Case of Canine Melioidosis.
Veterinary sciences. 6(4) [DOI] 10.3390/vetsci6040076. [PMID] 31547534.
2018
Burkholderia pseudomallei distribution in Australasia is linked to paleogeographic and anthropogenic history.
PloS one. 13(11) [DOI] 10.1371/journal.pone.0206845. [PMID] 30395628.
2018
Cross-reactivity of latex agglutination assay complicates the identification of Burkholderia pseudomallei from soil.
FEMS microbiology letters. 365(22) [DOI] 10.1093/femsle/fny256. [PMID] 30346523.
2018
Lipid A Remodeling Is a Pathoadaptive Mechanism That Impacts Lipopolysaccharide Recognition and Intracellular Survival of Burkholderia pseudomallei.
Infection and immunity. 86(10) [DOI] 10.1128/IAI.00360-18. [PMID] 30037795.
2018
Outer Membrane Vesicle Vaccines from Biosafe Surrogates Prevent Acute Lethal Glanders in Mice.
Vaccines. 6(1) [DOI] 10.3390/vaccines6010005. [PMID] 29320408.
2018
Seroepidemiology of Burkholderia pseudomallei, Etiologic Agent of Melioidosis, in the Ouest and Sud-Est Departments of Haiti.
The American journal of tropical medicine and hygiene. 99(5):1222-1228 [DOI] 10.4269/ajtmh.18-0352. [PMID] 30226137.
2018
Transcriptional and post-transcriptional regulation of PenA β-lactamase in acquired Burkholderia pseudomallei β-lactam resistance.
Scientific reports. 8(1) [DOI] 10.1038/s41598-018-28843-7. [PMID] 30006637.
2017
An avirulent Burkholderia pseudomallei ∆purM strain with atypical type B LPS: expansion of the toolkit for biosafe studies of melioidosis.
BMC microbiology. 17(1) [DOI] 10.1186/s12866-017-1040-4. [PMID] 28592242.
2017
Antibiotic Resistance Markers in Burkholderia pseudomallei Strain Bp1651 Identified by Genome Sequence Analysis.
Antimicrobial agents and chemotherapy. 61(6) [DOI] 10.1128/AAC.00010-17. [PMID] 28396541.
2017
AOAC SMPR 2016.010.
Journal of AOAC International. 100(1):261-265 [DOI] 10.5740/jaoacint.SMPR2016.010. [PMID] 28825553.
2017
Development of Immunoassays for Burkholderia pseudomallei Typical and Atypical Lipopolysaccharide Strain Typing.
The American journal of tropical medicine and hygiene. 96(2):358-367 [DOI] 10.4269/ajtmh.16-0308. [PMID] 27994103.
2017
Global and regional dissemination and evolution of Burkholderia pseudomallei.
Nature microbiology. 2 [DOI] 10.1038/nmicrobiol.2016.263. [PMID] 28112723.
2017
Multitarget Quantitative PCR Improves Detection and Predicts Cultivability of the Pathogen Burkholderia pseudomallei.
Applied and environmental microbiology. 83(8) [DOI] 10.1128/AEM.03212-16. [PMID] 28188208.
2017
Phylogeographic, Genomic, and Meropenem Susceptibility Analysis of Burkholderia Ubonensis
PLoS neglected tropical diseases. 11(9) [DOI] 10.1371/journal.pntd.0005928. [PMID] 28910350.
2017
Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling.
PLoS neglected tropical diseases. 11(4) [DOI] 10.1371/journal.pntd.0005571. [PMID] 28453531.
2016
Correction for Johnson et al., Complete Genome Sequences for 59 Burkholderia Isolates, Both Pathogenic and Near Neighbor.
Genome announcements. 4(2) [DOI] 10.1128/genomeA.00313-16. [PMID] 27103731.
2016
The Effects of Signal Erosion and Core Genome Reduction on the Identification of Diagnostic Markers.
mBio. 7(5) [DOI] 10.1128/mBio.00846-16. [PMID] 27651357.
2016
Two-Phase Bactericidal Mechanism of Silver Nanoparticles against Burkholderia pseudomallei.
PloS one. 11(12) [DOI] 10.1371/journal.pone.0168098. [PMID] 27977746.
2015
A Burkholderia pseudomallei colony variant necessary for gastric colonization.
mBio. 6(1) [DOI] 10.1128/mBio.02462-14. [PMID] 25650400.
2015
Complete Genome Sequence of Burkholderia cepacia Strain LO6.
Genome announcements. 3(3) [DOI] 10.1128/genomeA.00587-15. [PMID] 26067955.
2015
Direct detection of Burkholderia pseudomallei and biological factors in soil.
Transactions of the Royal Society of Tropical Medicine and Hygiene. 109(7):462-8 [DOI] 10.1093/trstmh/trv040. [PMID] 26048871.
2015
Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia.
PLoS neglected tropical diseases. 9(6) [DOI] 10.1371/journal.pntd.0003892. [PMID] 26121041.
2015
Melioidosis diagnostic workshop, 2013.
Emerging infectious diseases. 21(2) [DOI] 10.3201/eid2102.141045. [PMID] 25626057.
2015
Pangenome Analysis of Burkholderia pseudomallei: Genome Evolution Preserves Gene Order despite High Recombination Rates.
PloS one. 10(10) [DOI] 10.1371/journal.pone.0140274. [PMID] 26484663.
2014
Burkholderia pseudomallei isolates from Sarawak, Malaysian Borneo, are predominantly susceptible to aminoglycosides and macrolides.
Antimicrobial agents and chemotherapy. 58(1):162-6 [DOI] 10.1128/AAC.01842-13. [PMID] 24145517.
2014
Burkholderia pseudomallei type G in Western Hemisphere.
Emerging infectious diseases. 20(4):682-4 [DOI] 10.3201/eid2004.130960. [PMID] 24655932.
2014
Structural characterization of polysaccharides expressed by Burkholderia oklahomensis E0147.
Carbohydrate research. 386:68-72 [DOI] 10.1016/j.carres.2013.08.011. [PMID] 24491843.
2014
Variable virulence factors in Burkholderia pseudomallei (melioidosis) associated with human disease.
PloS one. 9(3) [DOI] 10.1371/journal.pone.0091682. [PMID] 24618705.
2013
Accurate and rapid identification of the Burkholderia pseudomallei near-neighbour, Burkholderia ubonensis, using real-time PCR.
PloS one. 8(8) [DOI] 10.1371/journal.pone.0071647. [PMID] 23967229.
2013
Complete Genome Sequence of the Encephalomyelitic Burkholderia pseudomallei Strain MSHR305.
Genome announcements. 1(4) [DOI] 10.1128/genomeA.00656-13. [PMID] 23969058.
2013
Genome Sequence of Burkholderia pseudomallei NCTC 13392.
Genome announcements. 1(3) [DOI] 10.1128/genomeA.00183-13. [PMID] 23704173.
2013
Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei.
PLoS neglected tropical diseases. 7(3) [DOI] 10.1371/journal.pntd.0002105. [PMID] 23556010.
2013
Within-host evolution of Burkholderia pseudomallei over a twelve-year chronic carriage infection.
mBio. 4(4) [DOI] 10.1128/mBio.00388-13. [PMID] 23860767.
2012
An objective approach for Burkholderia pseudomallei strain selection as challenge material for medical countermeasures efficacy testing.
Frontiers in cellular and infection microbiology. 2 [DOI] 10.3389/fcimb.2012.00120. [PMID] 23057010.
2012
Characterization of ceftazidime resistance mechanisms in clinical isolates of Burkholderia pseudomallei from Australia.
PloS one. 7(2) [DOI] 10.1371/journal.pone.0030789. [PMID] 22363490.
2012
Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens.
Journal of clinical microbiology. 50(6):2059-62 [DOI] 10.1128/JCM.06737-11. [PMID] 22442327.
2012
Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species.
BMC microbiology. 12 [DOI] 10.1186/1471-2180-12-250. [PMID] 23126230.
2012
Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications.
PloS one. 7(5) [DOI] 10.1371/journal.pone.0037723. [PMID] 22624061.
2012
Out of the ground: aerial and exotic habitats of the melioidosis bacterium Burkholderia pseudomallei in grasses in Australia.
Environmental microbiology. 14(8):2058-70 [DOI] 10.1111/j.1462-2920.2011.02671.x. [PMID] 22176696.
2012
The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharide.
PLoS neglected tropical diseases. 6(1) [DOI] 10.1371/journal.pntd.0001453. [PMID] 22235357.
2012
The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems.
Molecular microbiology. 84(3):516-29 [DOI] 10.1111/j.1365-2958.2012.08039.x. [PMID] 22435733.
2012
Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens.
Journal of microbiological methods. 88(1):179-81 [DOI] 10.1016/j.mimet.2011.10.023. [PMID] 22108495.
2011
Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.
PloS one. 6(12) [DOI] 10.1371/journal.pone.0029323. [PMID] 22195045.
2011
Epidemiological tracking and population assignment of the non-clonal bacterium, Burkholderia pseudomallei.
PLoS neglected tropical diseases. 5(12) [DOI] 10.1371/journal.pntd.0001381. [PMID] 22180792.
2011
Epidemiology and investigation of melioidosis, Southern Arizona.
Emerging infectious diseases. 17(7):1286-8 [DOI] 10.3201/eid1707.100661. [PMID] 21762589.
2011
Knockout and pullout recombineering for naturally transformable Burkholderia thailandensis and Burkholderia pseudomallei.
Nature protocols. 6(8):1085-104 [DOI] 10.1038/nprot.2011.346. [PMID] 21738123.
2011
Molecular characterization of clinical Burkholderia pseudomallei isolates from India.
The American journal of tropical medicine and hygiene. 85(1):121-3 [DOI] 10.4269/ajtmh.2011.11-0166. [PMID] 21734136.
2011
Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA.
PLoS neglected tropical diseases. 5(10) [DOI] 10.1371/journal.pntd.0001347. [PMID] 22028940.
2010
BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
PloS one. 5(11) [DOI] 10.1371/journal.pone.0015413. [PMID] 21103048.
2010
Characterization of the type III capsular polysaccharide produced by Burkholderia pseudomallei.
Journal of medical microbiology. 59(Pt 12):1403-1414 [DOI] 10.1099/jmm.0.022202-0. [PMID] 20724509.
2010
Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis.
PLoS pathogens. 6(1) [DOI] 10.1371/journal.ppat.1000725. [PMID] 20090837.
2009
Molecular basis of rare aminoglycoside susceptibility and pathogenesis of Burkholderia pseudomallei clinical isolates from Thailand.
PLoS neglected tropical diseases. 3(9) [DOI] 10.1371/journal.pntd.0000519. [PMID] 19771149.
2009
Phylogeographic reconstruction of a bacterial species with high levels of lateral gene transfer.
BMC biology. 7 [DOI] 10.1186/1741-7007-7-78. [PMID] 19922616.
2008
Genomic islands from five strains of Burkholderia pseudomallei.
BMC genomics. 9 [DOI] 10.1186/1471-2164-9-566. [PMID] 19038032.
2008
Survival of Burkholderia pseudomallei in water.
BMC research notes. 1 [DOI] 10.1186/1756-0500-1-11. [PMID] 18710531.
2008
The core and accessory genomes of Burkholderia pseudomallei: implications for human melioidosis.
PLoS pathogens. 4(10) [DOI] 10.1371/journal.ppat.1000178. [PMID] 18927621.
2007
A horizontal gene transfer event defines two distinct groups within Burkholderia pseudomallei that have dissimilar geographic distributions.
Journal of bacteriology. 189(24):9044-9 [PMID] 17933898.
View on: PubMed
2007
Yersinia pestis evolution on a small timescale: comparison of whole genome sequences from North America.
PloS one. 2(8) [PMID] 17712418.
View on: PubMed
2006
Genome-wide expression analysis of Burkholderia pseudomallei infection in a hamster model of acute melioidosis.
Infection and immunity. 74(10):5465-76 [PMID] 16988221.
View on: PubMed
2005
Genome-wide expression analysis of iron regulation in Burkholderia pseudomallei and Burkholderia mallei using DNA microarrays.
FEMS microbiology letters. 252(2):327-35 [PMID] 16242861.
View on: PubMed
2004
Contribution of gene loss to the pathogenic evolution of Burkholderia pseudomallei and Burkholderia mallei.
Infection and immunity. 72(7):4172-87 [PMID] 15213162.
View on: PubMed

Grants

Jul 2021 ACTIVE
Discovery of Bacteriophages that Mediate Antimicrobial Susceptibility Trough Efflux Pump Dependent Infection
Role: Principal Investigator
Funding: NORTHERN ARIZONA UNIVERSITY via DEFENSE THREAT REDUCTION AGENCY
Jun 2017 – May 2020
Membrane bound Burkholderia pseudomallei PenA beta-lactamase
Role: Faculty
Funding: NATL INST OF HLTH NIAID
Feb 2017 – May 2018
Molecular Surveillance of Ceftazidime (CAZ) Resistance in Melioidosis
Role: Principal Investigator
Funding: CRDF GLOBAL
Feb 2017 – May 2020
Eco-environmental signatures of danger to identify melioidosis-endemic hotzones.
Role: Principal Investigator
Funding: UNIV OF CALIFORNIA LOS ANGELES via DEFENSE THREAT REDUCTION AGENCY
Jan 2017 ACTIVE
Modulation of Burkholderia pseudomallei immune responses by human-like T cell epitopes.
Role: Principal Investigator
Funding: CUBRC via DEFENSE THREAT REDUCTION AGENCY
Jan 2015 – Sep 2015
Antigenic protein and lipopolysaccharide (LPS) signatures from Burkholderia pseudomallei and B. mallei towards specific biothreat detection strategies.
Role: Principal Investigator
Funding: NORTHERN ARIZONA UNIVERSITY via US DEPT OF HOMELAND SECURITY

Education

PhD
2000 · Liverpool School of Tropical Medicine, University of Liverpool

Teaching Profile

Courses Taught
2019-2021
VME6905 Problems in Veterinary Medical Sciences
2020-2021
VEM5571 Emerging Diseases
2016,2018-2019
VME6464 Molecular Pathogenesis
2018
GMS7980 Research for Doctoral Dissertation
2018
VME7979 Advanced Research

Contact Details

Phones:
Business:
(352) 294-8269
Emails:
Business:
tuanyok@ufl.edu