Bikash Sahay,
Research Associate Professor
Department:
Department of Infectious Diseases & Immunology
Business Phone:
(352) 294-4155
Business Email:
sahayb@ufl.edu
Teaching Profile
Courses Taught
2020
VME6464 Molecular Pathogenesis
2017-2018
VEM5131 Vet Molecular Biology
2021-2024
VEM5141 Vet Microbiology
2021-2023
VEM5110 Animal Systems 1
2024
VME6934 Topics in Veterinary Medical Sciences
Research Profile
The human gastrointestinal (GI) tract harbors a complex consortium of trillions of microbes, thousands of bacterial phylotypes, methanogenic archaea, and fungi colonizing the entire gut length, collectively referred to as the microbiota, produce 100-fold many gene products as the host. Although it is largely unexplored and underappreciated, the gut microbiota plays a critical role in health and disease states. My research focuses on identifying microbes and using them or their products to treat human ailments, including infectious origin.
Areas of Interest
- Cancer Immunology and Immunotherapy
- Gastrointestinal physiology and immunology (necrotizing enterocolitis)
- Immune Tolerance
- Immune-Mediated Disease
- Mucosal immunology
Open Researcher and Contributor ID (ORCID)
0000-0003-0905-733X
Publications
2024
Interferon-γ/IL-2 ELISpot and mRNA Responses to the SARS-CoV2, Feline Coronavirus Serotypes 1 (FCoV1), and FCoV2 Receptor Binding Domains by the T Cells from COVID-19-Vaccinated Humans and FCoV1-Infected Cats.
Methods in molecular biology (Clifton, N.J.).
2768:135-151
[DOI] 10.1007/978-1-0716-3690-9_9.
[PMID] 38502392.
2024
RNA aggregates harness the danger response for potent cancer immunotherapy.
Cell.
187(10):2521-2535.e21
[DOI] 10.1016/j.cell.2024.04.003.
[PMID] 38697107.
2023
Both Feline Coronavirus Serotypes 1 and 2 Infected Domestic Cats Develop Cross-Reactive Antibodies to SARS-CoV-2 Receptor Binding Domain: Its Implication to Pan-CoV Vaccine Development
Viruses.
15(4)
[DOI] 10.3390/v15040914.
[PMID] 37112894.
2023
mRNA aggregates harness danger response for potent cancer immunotherapy.
medRxiv : the preprint server for health sciences.
[DOI] 10.1101/2023.03.12.23287108.
[PMID] 36993772.
2022
Protective immunity enhanced Salmonella vaccine vectors delivering Helicobacter pylori antigens reduce H. pylori stomach colonization in mice.
Frontiers in immunology.
13
[DOI] 10.3389/fimmu.2022.1034683.
[PMID] 36466847.
2021
A Tryptophan-Deficient Diet Induces Gut Microbiota Dysbiosis and Increases Systemic Inflammation in Aged Mice
International Journal of Molecular Sciences.
22(9)
[DOI] 10.3390/ijms22095005.
[PMID] 34066870.
2021
Analysis of canine myeloid-derived suppressor cells (MDSCs) utilizing fluorescence-activated cell sorting, RNA protection mediums to yield quality RNA for single-cell RNA sequencing.
Veterinary immunology and immunopathology.
231
[DOI] 10.1016/j.vetimm.2020.110144.
[PMID] 33278779.
2021
Canine osteosarcoma checkpoint expression correlates with metastasis and T-cell infiltrate.
Veterinary immunology and immunopathology.
232
[DOI] 10.1016/j.vetimm.2020.110169.
[PMID] 33387703.
2021
Lactococcus lactis Delivery of Surface Layer Protein A Protects Mice from Colitis by Re-Setting Host Immune Repertoire.
Biomedicines.
9(9)
[DOI] 10.3390/biomedicines9091098.
[PMID] 34572293.
2021
Novel application of single-cell next-generation sequencing for determination of intratumoral heterogeneity of canine osteosarcoma cell lines
Journal of Veterinary Diagnostic Investigation.
33(2):1040638720985242-278
[DOI] 10.1177/1040638720985242.
[PMID] 33446089.
2020
COVID-19 Virulence in Aged Patients Might Be Impacted by the Host Cellular MicroRNAs Abundance/Profile.
Aging and disease.
11(3):509-522
[DOI] 10.14336/AD.2020.0428.
[PMID] 32489698.
2020
Protection against Borreliella burgdorferi infection mediated by a synthetically engineered DNA vaccine.
Human vaccines & immunotherapeutics.
16(9):2114-2122
[DOI] 10.1080/21645515.2020.1789408.
[PMID] 32783701.
2020
The Potential Contribution of Caveolin 1 to HIV Latent Infection
Pathogens.
9(11)
[DOI] 10.3390/pathogens9110896.
[PMID] 33121153.
2019
Characterization of myeloid-derived suppressor cells and cytokines GM-CSF, IL-10 and MCP-1 in dogs with malignant melanoma receiving a GD3-based immunotherapy.
Veterinary immunology and immunopathology.
216
[DOI] 10.1016/j.vetimm.2019.109912.
[PMID] 31446208.
2019
Immunogenicity and Efficacy of a Novel Multi-Antigenic Peptide Vaccine Based on Cross-Reactivity between Feline and Human Immunodeficiency Viruses
Viruses.
11(2)
[DOI] 10.3390/v11020136.
[PMID] 30717485.
2018
Dual-route targeted vaccine protects efficiently against botulinum neurotoxin A complex.
Vaccine.
36(1):155-164
[DOI] 10.1016/j.vaccine.2017.11.008.
[PMID] 29180028.
2018
Induction of Interleukin 10 by Borrelia burgdorferi Is Regulated by the Action of CD14-Dependent p38 Mitogen-Activated Protein Kinase and cAMP-Mediated Chromatin Remodeling.
Infection and immunity.
86(4)
[DOI] 10.1128/IAI.00781-17.
[PMID] 29311239.
2018
Lessons Learned in Developing a Commercial FIV Vaccine: The Immunity Required for an Effective HIV-1 Vaccine
Viruses.
10(5)
[DOI] 10.3390/v10050277.
[PMID] 29789450.
2018
Personalized Tumor RNA Loaded Lipid-Nanoparticles Prime the Systemic and Intratumoral Milieu for Response to Cancer Immunotherapy.
Nano letters.
18(10):6195-6206
[DOI] 10.1021/acs.nanolett.8b02179.
[PMID] 30259750.
2018
Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccine.
Methods in molecular biology (Clifton, N.J.).
1808:197-219
[DOI] 10.1007/978-1-4939-8567-8_18.
[PMID] 29956186.
2017
Commensal Propionibacterium strain UF1 mitigates intestinal inflammation via Th17 cell regulation
The Journal Of Clinical Investigation.
127(11):3970-3986
[DOI] 10.1172/JCI95376.
[PMID] 28945202.
2017
Conserved HIV Epitopes for an Effective HIV Vaccine.
Journal of clinical & cellular immunology.
8(4)
[DOI] 10.4172/2155-9899.1000518.
[PMID] 29226015.
2016
The role of the calcium-sensing receptor in gastrointestinal inflammation.
Seminars in cell & developmental biology.
49:44-51
[DOI] 10.1016/j.semcdb.2015.10.040.
[PMID] 26709005.
2015
Advancing the use of Lactobacillus acidophilus surface layer protein A for the treatment of intestinal disorders in humans.
Gut microbes.
6(6):392-7
[DOI] 10.1080/19490976.2015.1107697.
[PMID] 26647142.
2015
Gut dysbiosis is linked to hypertension.
Hypertension (Dallas, Tex. : 1979).
65(6):1331-40
[DOI] 10.1161/HYPERTENSIONAHA.115.05315.
[PMID] 25870193.
2015
Impact of Gastrointestinal Bacillus anthracis Infection on Hepatic B Cells
Toxins.
7(9):3805-3817
[DOI] 10.3390/toxins7093805.
[PMID] 26402706.
2015
SIGNR3-dependent immune regulation by Lactobacillus acidophilus surface layer protein A in colitis.
The EMBO journal.
34(7):881-95
[DOI] 10.15252/embj.201490296.
[PMID] 25666591.
2014
Colonic immune suppression, barrier dysfunction, and dysbiosis by gastrointestinal bacillus anthracis Infection.
PloS one.
9(6)
[DOI] 10.1371/journal.pone.0100532.
[PMID] 24945934.
2014
Epithelial CaSR deficiency alters intestinal integrity and promotes proinflammatory immune responses.
FEBS letters.
588(22):4158-66
[DOI] 10.1016/j.febslet.2014.05.007.
[PMID] 24842610.
2014
Impaired colonic B-cell responses by gastrointestinal Bacillus anthracis infection
The Journal of infectious diseases.
210(9):1499-507
[DOI] 10.1093/infdis/jiu280.
[PMID] 24829464.
2013
Activation of B cells by a dendritic cell-targeted oral vaccine.
Current pharmaceutical biotechnology.
14(10):867-77
[PMID] 24372255.
2013
Colonic immune stimulation by targeted oral vaccine.
PloS one.
8(1)
[DOI] 10.1371/journal.pone.0055143.
[PMID] 23383086.
2013
New generation of oral mucosal vaccines targeting dendritic cells.
Current opinion in chemical biology.
17(6):918-24
[DOI] 10.1016/j.cbpa.2013.06.013.
[PMID] 23835515.
2013
Targeting aberrant colon cancer-specific DNA methylation with lipoteichoic acid-deficient Lactobacillus acidophilus.
Gut microbes.
4(1):84-8
[DOI] 10.4161/gmic.22822.
[PMID] 23137966.
2012
The Toll of a TLR1 polymorphism in lyme disease: a tale of mice and men.
Arthritis and rheumatism.
64(5):1311-5
[DOI] 10.1002/art.34386.
[PMID] 22246662.
2011
CD14 signaling reciprocally controls collagen deposition and turnover to regulate the development of lyme arthritis.
The American journal of pathology.
178(2):724-34
[DOI] 10.1016/j.ajpath.2010.10.025.
[PMID] 21281805.
2011
Development of tolerogenic dendritic cells and regulatory T cells favors exponential bacterial growth and survival during early respiratory tularemia.
Journal of leukocyte biology.
90(3):493-507
[DOI] 10.1189/jlb.0411197.
[PMID] 21724804.
2011
Host-adaptation of Francisella tularensis alters the bacterium’s surface-carbohydrates to hinder effectors of innate and adaptive immunity.
PloS one.
6(7)
[DOI] 10.1371/journal.pone.0022335.
[PMID] 21799828.
2011
Phagosomal signaling by Borrelia burgdorferi in human monocytes involves Toll-like receptor (TLR) 2 and TLR8 cooperativity and TLR8-mediated induction of IFN-beta.
Proceedings of the National Academy of Sciences of the United States of America.
108(9):3683-8
[DOI] 10.1073/pnas.1013776108.
[PMID] 21321205.
2011
Reduced immune response to Borrelia burgdorferi in the absence of γδ T cells.
Infection and immunity.
79(10):3940-6
[DOI] 10.1128/IAI.00148-11.
[PMID] 21768278.
2009
CD14 signaling restrains chronic inflammation through induction of p38-MAPK/SOCS-dependent tolerance.
PLoS pathogens.
5(12)
[DOI] 10.1371/journal.ppat.1000687.
[PMID] 20011115.
2007
Development of an indirect ELISA for the detection of antibodies against Peste-des-petits-ruminants virus in small ruminants
Veterinary Research Communications.
31:355-364
2007
Mixed infection of peste des petits ruminants and orf on a goat farm in Shahjahanpur, India.
The Veterinary record.
160(12):410-2
[PMID] 17384295.
2007
Production and characterization of neutralizing monoclonal antibodies against haemagglutinin protein of peste des petits ruminants (PPR) vaccine virus
Journal of Applied Animal Research .
32:207-210
2006
Inhibition of Anatid Herpes Virus-1 replication by small interfering RNAs in cell culture system.
Virus research.
115(2):192-7
[PMID] 16199105.
2006
Toll-like receptor 2 is required for control of pulmonary infection with Francisella tularensis.
Infection and immunity.
74(6):3657-62
[PMID] 16714598.
Grants
Aug 2023
– Apr 2024
A Novel Immunological-Directed Live Biotherapy Product for Treating Ulcerative Colitis
Role: Principal Investigator
Funding: RISE THERAPEUTICS
via NATL INST OF HLTH NIDDK
Jan 2023
ACTIVE
A Novel Biology-Derived Microbiome Therapeutic to Treat Viral-Induced Acute Respiratory Distress Syndrome (ARDS)
Role: Principal Investigator
Funding: RISE THERAPEUTICS
via NATL INST OF HLTH NIAID
Mar 2022
ACTIVE
Mechanism of LSD1 Function and Its Therapeutic Application for Progressive Oral Malignancy
Role: Principal Investigator
Funding: BOSTON UNIV
via NATL INST OF HLTH NIDCR
May 2020
– Sep 2023
SBIR – A Novel Immunological Probiotic for Treating Inflammatory Bowel Disease.
Role: Principal Investigator
Funding: RISE THERAPEUTICS
via NATL INST OF HLTH NIDDK
Apr 2019
– Jul 2021
A Novel Probiotic Engineered for Enhanced Immunological Function for Treating Inflammatory Bowel Disease
Role: Principal Investigator
Funding: RISE THERAPEUTICS
via NATL INST OF HLTH NIAID
Nov 2018
– Mar 2019
DNA vaccine against Lyme disease
Role: Principal Investigator
Funding: INOVIO PHARMACEUTICALS INC
Jul 2018
– Jun 2020
A Novel Immunological Probiotic for Treating Inflammatory Bowel Disease
Role: Principal Investigator
Funding: RISE THERAPEUTICS
via NATL INST OF HLTH NIDDK
Feb 2018
– Dec 2019
MERCK/INTERVET ANIMAL CLINICAL VACCINE TRIAL
Role: Other
Funding: INTERVET
Jun 2017
– Apr 2019
CART Cells for Sarcoma
Role: Co-Investigator
Funding: Sarcoma Foundation of America
Education
Doctor of Philosophy
2005
·
Indian Veterinary Research Institute
Master of Veterinary Science
2001
·
Indian Veterinary Research Institute
Bachelor of Veterinary Science
1999
·
Bihar Veterinary College, Rajendra Agricultural University
Contact Details
Phones:
- Business:
- (352) 294-4155
Emails:
- Business:
- sahayb@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 110880
GAINESVILLE FL 32611