Bikash Sahay

Bikash Sahay,

CLIN AST PROF

Department: Department of Infectious Diseases & Immunology
Business Phone: (352) 294-4155
Business Email: sahayb@ufl.edu

Research Profile

The human gastrointestinal (GI) tract harbors a complex consortium of trillions of microbes, thousands of bacterial phylotypes, methanogenic archaea, and fungi colonizing the entire length of the gut. Collectively this is termed microbiota, which contains at least 100 times as many gene products as the host genome. Although it is largely unexplored and underappreciated, the gut microbiota plays a critical role in states of both health and disease. Accumulating evidence indicates that the gut microbiota is essential in energy metabolism, host intestinal immune function and is also crucial in developing several diseases, including diabetes, non-alcoholic fatty liver disease, inflammatory bowel diseases, and even cancer. My research focuses on developing strategic alterations in microbiota, which will, in turn, treat human ailments, including those of infectious origin.

Areas of Interest
  • Cancer Immunology and Immunotherapy
  • Gastrointestinal physiology and immunology (necrotizing enterocolitis)
  • Immune Tolerance
  • Immune-Mediated Disease
  • Mucosal immunology

Publications

2021
A Tryptophan-Deficient Diet Induces Gut Microbiota Dysbiosis and Increases Systemic Inflammation in Aged Mice.
International journal of molecular sciences. 22(9) [DOI] 10.3390/ijms22095005. [PMID] 34066870.
2021
Analysis of canine myeloid-derived suppressor cells (MDSCs) utilizing fluorescence-activated cell sorting, RNA protection mediums to yield quality RNA for single-cell RNA sequencing.
Veterinary immunology and immunopathology. 231 [DOI] 10.1016/j.vetimm.2020.110144. [PMID] 33278779.
2021
Canine osteosarcoma checkpoint expression correlates with metastasis and T-cell infiltrate.
Veterinary immunology and immunopathology. 232 [DOI] 10.1016/j.vetimm.2020.110169. [PMID] 33387703.
2021
Novel application of single-cell next-generation sequencing for determination of intratumoral heterogeneity of canine osteosarcoma cell lines
Journal of Veterinary Diagnostic Investigation. 33(2):1040638720985242-278 [DOI] 10.1177/1040638720985242. [PMID] 33446089.
2020
COVID-19 Virulence in Aged Patients Might Be Impacted by the Host Cellular MicroRNAs Abundance/Profile.
Aging and disease. 11(3):509-522 [DOI] 10.14336/AD.2020.0428. [PMID] 32489698.
2020
Protection against Borreliella burgdorferi infection mediated by a synthetically engineered DNA vaccine.
Human vaccines & immunotherapeutics. 16(9):2114-2122 [DOI] 10.1080/21645515.2020.1789408. [PMID] 32783701.
2020
The Potential Contribution of Caveolin 1 to HIV Latent Infection.
Pathogens (Basel, Switzerland). 9(11) [DOI] 10.3390/pathogens9110896. [PMID] 33121153.
2019
Characterization of myeloid-derived suppressor cells and cytokines GM-CSF, IL-10 and MCP-1 in dogs with malignant melanoma receiving a GD3-based immunotherapy.
Veterinary immunology and immunopathology. 216 [DOI] 10.1016/j.vetimm.2019.109912. [PMID] 31446208.
2019
Immunogenicity and Efficacy of a Novel Multi-Antigenic Peptide Vaccine Based on Cross-Reactivity between Feline and Human Immunodeficiency Viruses.
Viruses. 11(2) [DOI] 10.3390/v11020136. [PMID] 30717485.
2018
Dual-route targeted vaccine protects efficiently against botulinum neurotoxin A complex.
Vaccine. 36(1):155-164 [DOI] 10.1016/j.vaccine.2017.11.008. [PMID] 29180028.
2018
Induction of Interleukin 10 by Borrelia burgdorferi Is Regulated by the Action of CD14-Dependent p38 Mitogen-Activated Protein Kinase and cAMP-Mediated Chromatin Remodeling.
Infection and immunity. 86(4) [DOI] 10.1128/IAI.00781-17. [PMID] 29311239.
2018
Lessons Learned in Developing a Commercial FIV Vaccine: The Immunity Required for an Effective HIV-1 Vaccine.
Viruses. 10(5) [DOI] 10.3390/v10050277. [PMID] 29789450.
2018
Personalized Tumor RNA Loaded Lipid-Nanoparticles Prime the Systemic and Intratumoral Milieu for Response to Cancer Immunotherapy.
Nano letters. 18(10):6195-6206 [DOI] 10.1021/acs.nanolett.8b02179. [PMID] 30259750.
2018
Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccine.
Methods in molecular biology (Clifton, N.J.). 1808:197-219 [DOI] 10.1007/978-1-4939-8567-8_18. [PMID] 29956186.
2017
Commensal Propionibacterium strain UF1 mitigates intestinal inflammation via Th17 cell regulation
The Journal Of Clinical Investigation. 127(11):3970-3986 [DOI] 10.1172/JCI95376. [PMID] 28945202.
2017
Conserved HIV Epitopes for an Effective HIV Vaccine.
Journal of clinical & cellular immunology. 8(4) [DOI] 10.4172/2155-9899.1000518. [PMID] 29226015.
2016
The role of the calcium-sensing receptor in gastrointestinal inflammation.
Seminars in cell & developmental biology. 49:44-51 [DOI] 10.1016/j.semcdb.2015.10.040. [PMID] 26709005.
2015
Advancing the use of Lactobacillus acidophilus surface layer protein A for the treatment of intestinal disorders in humans.
Gut microbes. 6(6):392-7 [DOI] 10.1080/19490976.2015.1107697. [PMID] 26647142.
2015
Gut dysbiosis is linked to hypertension.
Hypertension (Dallas, Tex. : 1979). 65(6):1331-40 [DOI] 10.1161/HYPERTENSIONAHA.115.05315. [PMID] 25870193.
2015
Impact of Gastrointestinal Bacillus anthracis Infection on Hepatic B Cells.
Toxins. 7(9):3805-17 [DOI] 10.3390/toxins7093805. [PMID] 26402706.
2015
SIGNR3-dependent immune regulation by Lactobacillus acidophilus surface layer protein A in colitis.
The EMBO journal. 34(7):881-95 [DOI] 10.15252/embj.201490296. [PMID] 25666591.
2014
Colonic immune suppression, barrier dysfunction, and dysbiosis by gastrointestinal bacillus anthracis Infection.
PloS one. 9(6) [DOI] 10.1371/journal.pone.0100532. [PMID] 24945934.
2014
Epithelial CaSR deficiency alters intestinal integrity and promotes proinflammatory immune responses.
FEBS letters. 588(22):4158-66 [DOI] 10.1016/j.febslet.2014.05.007. [PMID] 24842610.
2014
Impaired colonic B-cell responses by gastrointestinal Bacillus anthracis infection
The Journal of infectious diseases. 210(9):1499-507 [DOI] 10.1093/infdis/jiu280. [PMID] 24829464.
2013
Activation of B cells by a dendritic cell-targeted oral vaccine.
Current pharmaceutical biotechnology. 14(10):867-77 [PMID] 24372255.
View on: PubMed
2013
Colonic immune stimulation by targeted oral vaccine.
PloS one. 8(1) [DOI] 10.1371/journal.pone.0055143. [PMID] 23383086.
2013
New generation of oral mucosal vaccines targeting dendritic cells.
Current opinion in chemical biology. 17(6):918-24 [DOI] 10.1016/j.cbpa.2013.06.013. [PMID] 23835515.
2013
Targeting aberrant colon cancer-specific DNA methylation with lipoteichoic acid-deficient Lactobacillus acidophilus.
Gut microbes. 4(1):84-8 [DOI] 10.4161/gmic.22822. [PMID] 23137966.
2012
The Toll of a TLR1 polymorphism in lyme disease: a tale of mice and men.
Arthritis and rheumatism. 64(5):1311-5 [DOI] 10.1002/art.34386. [PMID] 22246662.
2011
CD14 signaling reciprocally controls collagen deposition and turnover to regulate the development of lyme arthritis.
The American journal of pathology. 178(2):724-34 [DOI] 10.1016/j.ajpath.2010.10.025. [PMID] 21281805.
2011
Development of tolerogenic dendritic cells and regulatory T cells favors exponential bacterial growth and survival during early respiratory tularemia.
Journal of leukocyte biology. 90(3):493-507 [DOI] 10.1189/jlb.0411197. [PMID] 21724804.
2011
Host-adaptation of Francisella tularensis alters the bacterium’s surface-carbohydrates to hinder effectors of innate and adaptive immunity.
PloS one. 6(7) [DOI] 10.1371/journal.pone.0022335. [PMID] 21799828.
2011
Phagosomal signaling by Borrelia burgdorferi in human monocytes involves Toll-like receptor (TLR) 2 and TLR8 cooperativity and TLR8-mediated induction of IFN-beta.
Proceedings of the National Academy of Sciences of the United States of America. 108(9):3683-8 [DOI] 10.1073/pnas.1013776108. [PMID] 21321205.
2011
Reduced immune response to Borrelia burgdorferi in the absence of γδ T cells.
Infection and immunity. 79(10):3940-6 [DOI] 10.1128/IAI.00148-11. [PMID] 21768278.
2009
CD14 signaling restrains chronic inflammation through induction of p38-MAPK/SOCS-dependent tolerance.
PLoS pathogens. 5(12) [DOI] 10.1371/journal.ppat.1000687. [PMID] 20011115.
2006
Toll-like receptor 2 is required for control of pulmonary infection with Francisella tularensis.
Infection and immunity. 74(6):3657-62 [PMID] 16714598.
View on: PubMed

Grants

May 2020 ACTIVE
SBIR – A Novel Immunological Probiotic for Treating Inflammatory Bowel Disease.
Role: Principal Investigator
Funding: RISE THERAPEUTICS via NATL INST OF HLTH NIDDK
Apr 2019 – Jul 2021
A Novel Probiotic Engineered for Enhanced Immunological Function for Treating Inflammatory Bowel Disease
Role: Principal Investigator
Funding: RISE THERAPEUTICS via NATL INST OF HLTH NIAID
Nov 2018 – Mar 2019
DNA vaccine against Lyme disease
Role: Principal Investigator
Funding: INOVIO PHARMACEUTICALS INC
Jul 2018 – Jun 2020
A Novel Immunological Probiotic for Treating Inflammatory Bowel Disease
Role: Principal Investigator
Funding: RISE THERAPEUTICS via NATL INST OF HLTH NIDDK
Feb 2018 – Dec 2019
MERCK/INTERVET ANIMAL CLINICAL VACCINE TRIAL
Role: Other
Funding: INTERVET
Jun 2017 – Apr 2019
CART Cells for Sarcoma
Role: Co-Investigator
Funding: Sarcoma Foundation of America

Patents

Published November 2017
Propionibacterium Freudenreichii as a Probiotic for Infants
#US-2018-0199611-A1
Published October 2016
Lipoteichoic Acid From Lactobacilli as a Potent Immune Stimulatory Adjuvant for Vaccine Development
#US-2017-0035880-A1

Education

Doctor of Philosophy
2005 · Indian Veterinary Research Institute
Master of Veterinary Science
2001 · Indian Veterinary Research Institute
Bachelor of Veterinary Science
1999 · Bihar Veterinary College, Rajendra Agricultural University

Teaching Profile

Courses Taught
2020
VME6464 Molecular Pathogenesis
2017-2018
VEM5131 Vet Molecular Biology
2021
VEM5141 Vet Microbiology

Contact Details

Phones:
Business:
(352) 294-4155
Emails:
Business:
sahayb@ufl.edu