Bikash Sahay

Bikash Sahay,

CLIN AST PROF

Department: Department of Infectious Diseases & Immunology
Business Phone: (352) 294-4155
Business Email: sahayb@ufl.edu

Research Profile

Borrelia burgdorferi is the causative agent of Lyme disease in humans, and susceptibility to it varies widely within a population, a phenomenon also observed in mice. Different mouse strains were used to understand the differences and similarities between disease susceptibility and resistance in human populations. The C3H/HeN mouse strain is the most susceptible, whereas the C57BL/6 strain is most resistant to Lyme Borreliosis. There are several factors associated with the differential disease phenotype, and one of the major determining factors is the IL-10 production between the mice strains. C3H/HeN mice and macrophages (MΦ) isolated from them do not produce adequate IL-10 in response to B. burgdorferi; instead they produce genes belonging to interferon response factors. We found that C3H/HeN MΦ harbored less cAMP in their cytoplasm, and the addition of exogenous cAMP converts their phenotype closer to resistant MΦs isolated from C57/BL6 mice. Currently, my research focuses on the evaluation of the role of cAMP in modulating epigenetic landscape of disease susceptibility and resistance genes, including IL-10.

Publications

2021
Analysis of canine myeloid-derived suppressor cells (MDSCs) utilizing fluorescence-activated cell sorting, RNA protection mediums to yield quality RNA for single-cell RNA sequencing.
Veterinary immunology and immunopathology. 231 [DOI] 10.1016/j.vetimm.2020.110144. [PMID] 33278779.
2021
Canine osteosarcoma checkpoint expression correlates with metastasis and T-cell infiltrate.
Veterinary immunology and immunopathology. 232 [DOI] 10.1016/j.vetimm.2020.110169. [PMID] 33387703.
2021
Novel application of single-cell next-generation sequencing for determination of intratumoral heterogeneity of canine osteosarcoma cell lines
Journal of Veterinary Diagnostic Investigation. [DOI] 10.1177/1040638720985242. [PMID] 33446089.
2020
COVID-19 Virulence in Aged Patients Might Be Impacted by the Host Cellular MicroRNAs Abundance/Profile.
Aging and disease. 11(3):509-522 [DOI] 10.14336/AD.2020.0428. [PMID] 32489698.
2020
Protection against Borreliella burgdorferi infection mediated by a synthetically engineered DNA vaccine.
Human vaccines & immunotherapeutics. 16(9):2114-2122 [DOI] 10.1080/21645515.2020.1789408. [PMID] 32783701.
2020
The Potential Contribution of Caveolin 1 to HIV Latent Infection.
Pathogens (Basel, Switzerland). 9(11) [DOI] 10.3390/pathogens9110896. [PMID] 33121153.
2019
Characterization of myeloid-derived suppressor cells and cytokines GM-CSF, IL-10 and MCP-1 in dogs with malignant melanoma receiving a GD3-based immunotherapy.
Veterinary immunology and immunopathology. 216 [DOI] 10.1016/j.vetimm.2019.109912. [PMID] 31446208.
2019
Immunogenicity and Efficacy of a Novel Multi-Antigenic Peptide Vaccine Based on Cross-Reactivity between Feline and Human Immunodeficiency Viruses.
Viruses. 11(2) [DOI] 10.3390/v11020136. [PMID] 30717485.
2018
Dual-route targeted vaccine protects efficiently against botulinum neurotoxin A complex.
Vaccine. 36(1):155-164 [DOI] 10.1016/j.vaccine.2017.11.008. [PMID] 29180028.
2018
Induction of Interleukin 10 by Borrelia burgdorferi Is Regulated by the Action of CD14-Dependent p38 Mitogen-Activated Protein Kinase and cAMP-Mediated Chromatin Remodeling.
Infection and immunity. 86(4) [DOI] 10.1128/IAI.00781-17. [PMID] 29311239.
2018
Lessons Learned in Developing a Commercial FIV Vaccine: The Immunity Required for an Effective HIV-1 Vaccine.
Viruses. 10(5) [DOI] 10.3390/v10050277. [PMID] 29789450.
2018
Personalized Tumor RNA Loaded Lipid-Nanoparticles Prime the Systemic and Intratumoral Milieu for Response to Cancer Immunotherapy.
Nano letters. 18(10):6195-6206 [DOI] 10.1021/acs.nanolett.8b02179. [PMID] 30259750.
2018
Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccine.
Methods in molecular biology (Clifton, N.J.). 1808:197-219 [DOI] 10.1007/978-1-4939-8567-8_18. [PMID] 29956186.
2017
Commensal Propionibacterium strain UF1 mitigates intestinal inflammation via Th17 cell regulation
The Journal Of Clinical Investigation. 127(11):3970-3986 [DOI] 10.1172/JCI95376. [PMID] 28945202.
2017
Conserved HIV Epitopes for an Effective HIV Vaccine.
Journal of clinical & cellular immunology. 8(4) [DOI] 10.4172/2155-9899.1000518. [PMID] 29226015.
2016
The role of the calcium-sensing receptor in gastrointestinal inflammation.
Seminars in cell & developmental biology. 49:44-51 [DOI] 10.1016/j.semcdb.2015.10.040. [PMID] 26709005.
2015
Advancing the use of Lactobacillus acidophilus surface layer protein A for the treatment of intestinal disorders in humans.
Gut microbes. 6(6):392-7 [DOI] 10.1080/19490976.2015.1107697. [PMID] 26647142.
2015
Gut dysbiosis is linked to hypertension.
Hypertension (Dallas, Tex. : 1979). 65(6):1331-40 [DOI] 10.1161/HYPERTENSIONAHA.115.05315. [PMID] 25870193.
2015
Impact of Gastrointestinal Bacillus anthracis Infection on Hepatic B Cells.
Toxins. 7(9):3805-17 [DOI] 10.3390/toxins7093805. [PMID] 26402706.
2015
SIGNR3-dependent immune regulation by Lactobacillus acidophilus surface layer protein A in colitis.
The EMBO journal. 34(7):881-95 [DOI] 10.15252/embj.201490296. [PMID] 25666591.
2014
Colonic immune suppression, barrier dysfunction, and dysbiosis by gastrointestinal bacillus anthracis Infection.
PloS one. 9(6) [DOI] 10.1371/journal.pone.0100532. [PMID] 24945934.
2014
Epithelial CaSR deficiency alters intestinal integrity and promotes proinflammatory immune responses.
FEBS letters. 588(22):4158-66 [DOI] 10.1016/j.febslet.2014.05.007. [PMID] 24842610.
2014
Impaired colonic B-cell responses by gastrointestinal Bacillus anthracis infection
The Journal of infectious diseases. 210(9):1499-507 [DOI] 10.1093/infdis/jiu280. [PMID] 24829464.
2013
Activation of B cells by a dendritic cell-targeted oral vaccine.
Current pharmaceutical biotechnology. 14(10):867-77 [PMID] 24372255.
View on: PubMed
2013
Colonic immune stimulation by targeted oral vaccine.
PloS one. 8(1) [DOI] 10.1371/journal.pone.0055143. [PMID] 23383086.
2013
New generation of oral mucosal vaccines targeting dendritic cells.
Current opinion in chemical biology. 17(6):918-24 [DOI] 10.1016/j.cbpa.2013.06.013. [PMID] 23835515.
2013
Targeting aberrant colon cancer-specific DNA methylation with lipoteichoic acid-deficient Lactobacillus acidophilus.
Gut microbes. 4(1):84-8 [DOI] 10.4161/gmic.22822. [PMID] 23137966.
2012
The Toll of a TLR1 polymorphism in lyme disease: a tale of mice and men.
Arthritis and rheumatism. 64(5):1311-5 [DOI] 10.1002/art.34386. [PMID] 22246662.
2011
CD14 signaling reciprocally controls collagen deposition and turnover to regulate the development of lyme arthritis.
The American journal of pathology. 178(2):724-34 [DOI] 10.1016/j.ajpath.2010.10.025. [PMID] 21281805.
2011
Development of tolerogenic dendritic cells and regulatory T cells favors exponential bacterial growth and survival during early respiratory tularemia.
Journal of leukocyte biology. 90(3):493-507 [DOI] 10.1189/jlb.0411197. [PMID] 21724804.
2011
Host-adaptation of Francisella tularensis alters the bacterium’s surface-carbohydrates to hinder effectors of innate and adaptive immunity.
PloS one. 6(7) [DOI] 10.1371/journal.pone.0022335. [PMID] 21799828.
2011
Phagosomal signaling by Borrelia burgdorferi in human monocytes involves Toll-like receptor (TLR) 2 and TLR8 cooperativity and TLR8-mediated induction of IFN-beta.
Proceedings of the National Academy of Sciences of the United States of America. 108(9):3683-8 [DOI] 10.1073/pnas.1013776108. [PMID] 21321205.
2011
Reduced immune response to Borrelia burgdorferi in the absence of γδ T cells.
Infection and immunity. 79(10):3940-6 [DOI] 10.1128/IAI.00148-11. [PMID] 21768278.
2009
CD14 signaling restrains chronic inflammation through induction of p38-MAPK/SOCS-dependent tolerance.
PLoS pathogens. 5(12) [DOI] 10.1371/journal.ppat.1000687. [PMID] 20011115.
2006
Toll-like receptor 2 is required for control of pulmonary infection with Francisella tularensis.
Infection and immunity. 74(6):3657-62 [PMID] 16714598.
View on: PubMed

Grants

May 2020 ACTIVE
SBIR – A Novel Immunological Probiotic for Treating Inflammatory Bowel Disease.
Role: Principal Investigator
Funding: RISE THERAPEUTICS via NATL INST OF HLTH NIDDK
Apr 2019 ACTIVE
A Novel Probiotic Engineered for Enhanced Immunological Function for Treating Inflammatory Bowel Disease
Role: Principal Investigator
Funding: RISE THERAPEUTICS via NATL INST OF HLTH NIAID
Nov 2018 – Mar 2019
DNA vaccine against Lyme disease
Role: Principal Investigator
Funding: INOVIO PHARMACEUTICALS INC
Jul 2018 – Jun 2020
A Novel Immunological Probiotic for Treating Inflammatory Bowel Disease
Role: Principal Investigator
Funding: RISE THERAPEUTICS via NATL INST OF HLTH NIDDK
Feb 2018 – Dec 2019
MERCK/INTERVET ANIMAL CLINICAL VACCINE TRIAL
Role: Other
Funding: INTERVET
Jun 2017 – Apr 2019
CART Cells for Sarcoma
Role: Co-Investigator
Funding: Sarcoma Foundation of America

Patents

Published November 2017
Propionibacterium Freudenreichii as a Probiotic for Infants
#US-2018-0199611-A1
Published October 2016
Lipoteichoic Acid From Lactobacilli as a Potent Immune Stimulatory Adjuvant for Vaccine Development
#US-2017-0035880-A1

Education

Doctor of Philosophy
2005 · Indian Veterinary Research Institute
Master of Veterinary Science
2001 · Indian Veterinary Research Institute
Bachelor of Veterinary Science
1999 · Bihar Veterinary College, Rajendra Agricultural University

Teaching Profile

Courses Taught
2020
VME6464 Molecular Pathogenesis
2017-2018
VEM5131 Vet Molecular Biology

Contact Details

Phones:
Business:
(352) 294-4155
Emails:
Business:
sahayb@ufl.edu

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