Subhashinie Kariyawasam

Subhashinie Kariyawasam, BVSc, PhD, DACVM, DACPV, DACVPM, DABMM

Chair, Department Of Comparative, Diagnostic, And Population Medicine; Professor, Microbiology

Department: Department of Comparative, Diagnostic & Population Medicine
Business Phone: (352) 294-8794
Business Email: skariyawasam@ufl.edu

Research Profile

Veterinary diagnostic microbiology; pathogenomics of Escherichia coli and Salmonella; emergence of antimicrobial resistance; bacterial pathogenesis.

Publications

2021
Complete Genome Sequence of Campylobacter hepaticus Strain UF2019SK1, Isolated from a Commercial Layer Flock in the United States.
Microbiology resource announcements. 10(12) [DOI] 10.1128/MRA.01446-20. [PMID] 33766908.
2021
Lactococcus lactis Delivery of Surface Layer Protein A Protects Mice from Colitis by Re-Setting Host Immune Repertoire.
Biomedicines. 9(9) [DOI] 10.3390/biomedicines9091098. [PMID] 34572293.
2021
Prevalence and distribution of multilocus sequence types of Staphylococcus aureus isolated from bulk tank milk and cows with mastitis in Pennsylvania
PLoS One. 16(3) [DOI] 10.1371/journal.pone.0248528. [PMID] 33711052.
2020
Comparison of Antimicrobial Resistance and Pan-Genome of Clinical and Non-Clinical Enterococcus cecorum from Poultry Using Whole-Genome Sequencing.
Foods (Basel, Switzerland). 9(6) [DOI] 10.3390/foods9060686. [PMID] 32466367.
2019
Complete Genome Sequences of 20 Nontyphoidal Salmonella Isolates from Rwanda
Microbiology resource announcements. 8(12):e00016-e00019 [DOI] 10.1128/MRA.00016-19. [PMID] 30938694.
2019
Detection of CTX-M-1 extended-spectrum beta-lactamase among ceftiofur-resistant Salmonella enterica clinical isolates of poultry.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 31(5):681-687 [DOI] 10.1177/1040638719864384. [PMID] 31342869.
2019
The Escherichia coli O157:H7 carbon starvation-inducible lipoprotein Slp contributes to initial adherence in vitro via the human polymeric immunoglobulin receptor.
PloS one. 14(6) [DOI] 10.1371/journal.pone.0216791. [PMID] 31188867.
2018
High-Resolution Identification of Multiple Salmonella Serovars in a Single Sample by Using CRISPR-SeroSeq.
Applied and environmental microbiology. 84(21) [DOI] 10.1128/AEM.01859-18. [PMID] 30170999.
2018
Prevalence of Group I Salmonella Kentucky in domestic food animals from Pennsylvania and overlap with human clinical CRISPR sequence types.
Zoonoses and public health. 65(7):831-837 [DOI] 10.1111/zph.12506. [PMID] 30014599.
2018
Shigatoxigenic and atypical enteropathogenic Escherichia coli in fish for human consumption.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. 49(4):936-941 [DOI] 10.1016/j.bjm.2018.02.013. [PMID] 29891415.
2018
The efficacy of self-disinfecting bedrail covers in an intensive care unit.
American journal of infection control. 46(4):417-419 [DOI] 10.1016/j.ajic.2017.09.026. [PMID] 29162291.
2017
A Retrospective Study of Salmonella Enteritidis Isolated from Commercial Layer Flocks.
Avian diseases. 61(3):330-334 [DOI] 10.1637/11590-011817-RegR. [PMID] 28956999.
2017
Captive and free-living urban pigeons (Columba livia) from Brazil as carriers of multidrug-resistant pathogenic Escherichia coli.
Veterinary journal (London, England : 1997). 219:65-67 [DOI] 10.1016/j.tvjl.2016.12.015. [PMID] 28093116.
2017
Evaluation of Three Bacterial Identification Systems for Species Identification of Bacteria Isolated from Bovine Mastitis and Bulk Tank Milk Samples.
Foodborne pathogens and disease. 14(3):177-187 [DOI] 10.1089/fpd.2016.2222. [PMID] 28128639.
2016
Comparison of antimicrobial resistant genes in chicken gut microbiome grown on organic and conventional diet.
Veterinary and animal science. 1-2:9-14 [DOI] 10.1016/j.vas.2016.07.001. [PMID] 32734018.
2016
Growth in Egg Yolk Enhances Salmonella Enteritidis Colonization and Virulence in a Mouse Model of Human Colitis.
PloS one. 11(3) [DOI] 10.1371/journal.pone.0150258. [PMID] 26939126.
2016
Longitudinal Monitoring of Successive Commercial Layer Flocks for Salmonella enterica Serovar Enteritidis.
Foodborne pathogens and disease. 13(11):618-625 [PMID] 27792449.
View on: PubMed
2016
Surveillance for Pasteurella multocida in Ring-Necked Pheasants (Phasianus colchicus) After an Outbreak of Avian Cholera and Apparently Successful Antibiotic Treatment.
Avian diseases. 60(1):87-9 [DOI] 10.1637/11301-101315-Case.1. [PMID] 26953951.
2016
Whole genome sequencing of diverse Shiga toxin-producing and non-producing Escherichia coli strains reveals a variety of virulence and novel antibiotic resistance plasmids.
Plasmid. 83:8-11 [DOI] 10.1016/j.plasmid.2015.12.001. [PMID] 26746359.
2015
Characterizing the pathotype of neonatal meningitis causing Escherichia coli (NMEC).
BMC microbiology. 15 [DOI] 10.1186/s12866-015-0547-9. [PMID] 26467858.
2015
Complete Genome Sequence of Escherichia coli Strain RS218 (O18:H7:K1), Associated with Neonatal Meningitis.
Genome announcements. 3(4) [DOI] 10.1128/genomeA.00804-15. [PMID] 26205862.
2015
Genome Sequences of Two Strains of Salmonella enterica Serovar Enteritidis Isolated from Shell Eggs.
Genome announcements. 3(5) [DOI] 10.1128/genomeA.00954-15. [PMID] 26358589.
2015
Histiocytic typhlocolitis in two colony Beagle dogs.
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. 67(2):219-21 [DOI] 10.1016/j.etp.2014.10.004. [PMID] 25467750.
2015
Risk Factors Associated With Salmonella in Laying Hen Farms: Systematic Review of Observational Studies.
Avian diseases. 59(2):291-302 [DOI] 10.1637/10997-120214-Reg. [PMID] 26473681.
2014
Acute septicemia caused by Streptococcus gallolyticus subsp. pasteurianus in turkey poults.
Avian diseases. 58(2):318-22 [PMID] 25055641.
View on: PubMed
2014
Advances in vaccination against avian pathogenic Escherichia coli respiratory disease: potentials and limitations.
Veterinary microbiology. 172(1-2):13-22 [DOI] 10.1016/j.vetmic.2014.04.019. [PMID] 24878325.
2014
An experimental infection model for Escherichia coli egg peritonitis in layer chickens.
Avian diseases. 58(1):25-33 [PMID] 24758109.
View on: PubMed
2014
An experimental infection model for Escherichia coli egg peritonitis in layer chickens.
Avian diseases. 58(3) [PMID] 25518426.
View on: PubMed
2014
Complete nucleotide sequence of pRS218, a large virulence plasmid, that augments pathogenic potential of meningitis-associated Escherichia coli strain RS218.
BMC microbiology. 14 [DOI] 10.1186/s12866-014-0203-9. [PMID] 25164788.
2014
Innate immunity to recombinant QseC, a bacterial adrenergic receptor, may regulate expression of virulence genes of avian pathogenic Escherichia coli.
Veterinary microbiology. 171(1-2):236-41 [DOI] 10.1016/j.vetmic.2014.03.024. [PMID] 24793502.
2014
Interdigitated silver-polymer-based antibacterial surface system activated by oligodynamic iontophoresis – an empirical characterization study.
Biomedical microdevices. 16(1):1-10 [DOI] 10.1007/s10544-013-9800-x. [PMID] 23917746.
2013
A novel two-component signaling system facilitates uropathogenic Escherichia coli’s ability to exploit abundant host metabolites.
PLoS pathogens. 9(6) [DOI] 10.1371/journal.ppat.1003428. [PMID] 23825943.
2013
Antibiotic Resistance in Salmonella enterica Serovar Typhimurium Associates with CRISPR Sequence Type.
Antimicrobial agents and chemotherapy. 57(9):4282-4289 [DOI] 10.1128/AAC.00913-13. [PMID] 23796925.
2013
Fatal pneumonia caused by Extraintestinal Pathogenic Escherichia coli (ExPEC) in a juvenile cat recovered from an animal hoarding incident.
Veterinary microbiology. 167(3-4):704-7 [DOI] 10.1016/j.vetmic.2013.08.015. [PMID] 24041770.
2012
Fingerprinting of poultry isolates of Enterococcus cecorum using three molecular typing methods.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 24(6):1166-71 [DOI] 10.1177/1040638712463563. [PMID] 23104952.
2012
Leukocyte transcriptome from chickens infected with avian pathogenic Escherichia coli identifies pathways associated with resistance
. 2:44-53
2012
Leukocyte transcriptome from chickens infected with avian pathogenic Escherichia coli identifies pathways associated with resistance.
Results in immunology. 2:44-53 [DOI] 10.1016/j.rinim.2012.02.003. [PMID] 24371566.
2012
Prevalence of avian-pathogenic Escherichia coli strain O1 genomic islands among extraintestinal and commensal E. coli isolates.
Journal of bacteriology. 194(11):2846-53 [DOI] 10.1128/JB.06375-11. [PMID] 22467781.
2012
Recombinant Iss as a potential vaccine for avian colibacillosis.
Avian diseases. 56(1):192-9 [PMID] 22545546.
View on: PubMed
2012
Strong concordance between transcriptomic patterns of spleen and peripheral blood leukocytes in response to avian pathogenic Escherichia coli infection.
Avian diseases. 56(4):732-6 [PMID] 23397846.
View on: PubMed
2012
tkt1, located on a novel pathogenicity island, is prevalent in avian and human extraintestinal pathogenic Escherichia coli.
BMC microbiology. 12 [DOI] 10.1186/1471-2180-12-51. [PMID] 22471764.
2011
Antimicrobial susceptibility of Arcanobacterium pyogenes isolated from the lungs of white-tailed deer (Odocoileus virginianus) with pneumonia.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 23(5):1009-13 [DOI] 10.1177/1040638711416618. [PMID] 21908365.
2011
Development of a real-time polymerase chain reaction assay for detection of Actinobacillus suis in porcine lung.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 23(5):885-9 [DOI] 10.1177/1040638711416854. [PMID] 21908342.
2011
In vitro antimicrobial inhibition of Mycoplasma bovis isolates submitted to the Pennsylvania Animal Diagnostic Laboratory using flow cytometry and a broth microdilution method.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 23(3):547-51 [DOI] 10.1177/1040638711404155. [PMID] 21908288.
2011
Micro-scale fabrication and characterization of a silver-polymer-based electrically activated antibacterial surface.
Biofabrication. 3(1) [DOI] 10.1088/1758-5082/3/1/015003. [PMID] 21245523.
2011
papA gene of avian pathogenic Escherichia coli.
Avian diseases. 55(4):532-8 [PMID] 22312970.
View on: PubMed
2011
Spleen transcriptome response to infection with avian pathogenic Escherichia coli in broiler chickens.
BMC genomics. 12 [DOI] 10.1186/1471-2164-12-469. [PMID] 21951686.
2011
Subtyping Salmonella enterica serovar enteritidis isolates from different sources by using sequence typing based on virulence genes and clustered regularly interspaced short palindromic repeats (CRISPRs).
Applied and environmental microbiology. 77(13):4520-6 [DOI] 10.1128/AEM.00468-11. [PMID] 21571881.
2010
AatA is a novel autotransporter and virulence factor of avian pathogenic Escherichia coli.
Infection and immunity. 78(3):898-906 [DOI] 10.1128/IAI.00513-09. [PMID] 20028805.
2010
Avian-pathogenic Escherichia coli strains are similar to neonatal meningitis E. coli strains and are able to cause meningitis in the rat model of human disease.
Infection and immunity. 78(8):3412-9 [DOI] 10.1128/IAI.00347-10. [PMID] 20515929.
2010
Sequence analysis and characterization of a transferable hybrid plasmid encoding multidrug resistance and enabling zoonotic potential for extraintestinal Escherichia coli.
Infection and immunity. 78(5):1931-42 [DOI] 10.1128/IAI.01174-09. [PMID] 20160015.
2009
Comparison of conventional direct and enrichment culture methods for Erysipelothrix spp. from experimentally and naturally infected swine.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 21(6):863-8 [PMID] 19901291.
View on: PubMed
2009
Comparison of genotypes of Escherichia coli strains carrying F18ab and F18ac fimbriae from pigs.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 21(3):359-64 [PMID] 19407090.
View on: PubMed
2009
Examination of the source and extended virulence genotypes of Escherichia coli contaminating retail poultry meat.
Foodborne pathogens and disease. 6(6):657-67 [DOI] 10.1089/fpd.2009.0266. [PMID] 19580453.
2009
Pap mutant of avian pathogenic Escherichia coli O1, an O1:K1:H7 strain, is attenuated in vivo.
Avian diseases. 53(2):255-60 [PMID] 19630233.
View on: PubMed
2009
Systemic Conidiobolus incongruus infection and hypertrophic osteopathy in a white-tailed deer (Odocoileus virginianus).
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 21(1):167-70 [PMID] 19139524.
View on: PubMed
2007
Common and specific genomic sequences of avian and human extraintestinal pathogenic Escherichia coli as determined by genomic subtractive hybridization.
BMC microbiology. 7 [PMID] 17760980.
View on: PubMed
2007
The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes.
Journal of bacteriology. 189(8):3228-36 [PMID] 17293413.
View on: PubMed
2006
Occurrence of pathogenicity island I(APEC-O1) genes among Escherichia coli implicated in avian colibacillosis.
Avian diseases. 50(3):405-10 [PMID] 17039841.
View on: PubMed
2006
The pap operon of avian pathogenic Escherichia coli strain O1:K1 is located on a novel pathogenicity island.
Infection and immunity. 74(1):744-9 [PMID] 16369033.
View on: PubMed
2006
Unique DNA sequences of avian pathogenic Escherichia coli isolates as determined by genomic suppression subtractive hybridization.
FEMS microbiology letters. 262(2):193-200 [PMID] 16923075.
View on: PubMed

Grants

Jan 2021 ACTIVE
Translating a discovery into improving food safety: a novel poultry Salmonella vaccine and diagnostic methodology to control foodborne salmonellosis
Role: Principal Investigator
Funding: US DEPT OF AG NATL INST OF FOOD AND AG

Education

DABMM
2020 · American Board of Medical Microbiology
DACVPM
2016 · American College of Veterinary Preventive Medicine
DACVM
2010 · American College of Poultry Veterinarians
DACVM
2006 · American College of Veterinary Microbiology
PhD
2003 · University of Guelph
BVSc
1992 · University of Peradeniya

Teaching Profile

Courses Taught
2019-2021
VEM5144 Lg An Appl Vet Micro
2020-2021
VEM5141 Vet Microbiology
2020-2021
VEM5313 Poultry Diseases
2019
VEM5991 Clkship Indiv Invest
2021
VEM5110 Animal Systems 1

Contact Details

Phones:
Business:
(352) 294-8794
Emails: