Cleverson De Souza

Cleverson De Souza,

Associate Professor – Clinical Pathology

Department: Department of Comparative, Diagnostic & Population Medicine
Business Phone: (352) 294-4153
Business Email: cdesouza@ufl.edu

About Cleverson De Souza

Dr. Cleverson de Souza earned his Doctor of Veterinary Medicine (DVM) degree from Universidade Federal do Paraná in 1999. He completed a two-year residency in veterinary clinical pathology at São Paulo State University (UNESP-Botucatu), followed by a Master of Science in Clinical Pathology from the Federal University of Santa Maria. After serving as faculty in veterinary clinical pathology at the University of Passo Fundo, Dr. Souza joined the research team led by Dr. Douglas J. Weiss at the University of Minnesota, where he investigated the molecular pathogenesis of Mycobacterium avium subspecies paratuberculosis. While at Minnesota, he also completed an accelerated residency in clinical pathology, achieving board certification from the American College of Veterinary Pathologists (ACVP).

Between 2009 and 2012, Dr. Souza taught General Pathology to medical and dental students at several Brazilian institutions, including the University of Fortaleza, UniChristus, and the Federal University of Ceará. He then joined Washington State University (WSU) in 2013, where he dedicated a decade of service, earning tenure as an Associate Professor. At WSU, Dr. Souza made contributions to clinical diagnostics, innovative educational practices, and research initiatives.

Currently, Dr. Souza is an Associate Professor in the Department of Comparative, Diagnostic, and Population Medicine at the University of Florida. His professional activities remain deeply rooted in providing high-quality diagnostic service, developing and employing cutting-edge teaching methodologies, and pursuing impactful research dedicated to advancing veterinary medicine and enhancing animal health globally.

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Teaching Profile

Courses Taught
2023-2025
VEM5221 Intro Clinic Pathol
2023-2025
VEM5854 Clinical Pathology
2023-2024
VEM5855 Clkship Clin Pathol
2025
VEM5222C Cytodiagnos Vet Pract
Teaching Philosophy
My teaching philosophy centers on evidence-based strategies designed to foster deep understanding and lasting mastery. I strive to create an interactive, inclusive learning environment that encourages active participation, reflective thinking, and independent inquiry through real-world applications and collaborative activities. In the near term, I plan to integrate AI-powered tools to tailor learning experiences to individual needs, connect new ideas with prior knowledge, provide prompt and actionable feedback, and deliver clear insights into student progress. As these methods mature, I aim to further leverage AI to enhance students’ metacognitive skills, empowering them to better assess their own understanding, optimize their study techniques, and take ownership of their educational journeys. Ultimately, my objective is to equip students with the confidence, technological competence, and resilience necessary for lifelong learning, nurturing a growth mindset built upon curiosity, perseverance, and continuous improvement.

Board Certifications

  • Clinical Pathology
    American College of Veterinary Pathologists (ACVP)

Research Profile

Dr. Souza’s research program integrates foundational scientific research, practical clinical applications, and innovative educational methods. Current research includes:

Basic Research: Vaccine Development: Developing peptide-based vaccines targeting Mycobacterium avium subspecies paratuberculosis (MAP) in cattle to improve disease prevention and management.

Clinical Research: (1) Validating monoclonal antibodies to enhance the detection and classification of canine lymphomas. (2) AI-Enhanced Diagnostics: Applying machine learning and deep learning techniques to improve accuracy and speed of diagnosing canine neoplasia.

Educational Research: Experimenting with AI-driven chatbot systems to create personalized educational experiences, improve knowledge retention, and develop critical thinking skills in veterinary students, aiming to better prepare them for clinical practice.

Areas of Interest
  • Artificial Intelligence
  • Clinical pathology of mammals
  • Vaccine immunology

Publications

Academic Articles
2025
Major membrane protein of Mycobacteriumavium subp. paratuberculosis activates immune and autophagic pathways in bovine monocyte-derived macrophages  
Veterinary Immunology and Immunopathology. [DOI] https://doi.org/10.1016/j.vetimm.2025.110901 .
2025
Update on development of monoclonal antibodies for use in clinical flow cytometry and research in dogs.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. [DOI] 10.1177/10406387251315530. [PMID] 39910737.
2024
Cytologic features of canine melanotroph and corticotroph pituitary adenomas.
Veterinary clinical pathology. 53(1):99-103 [DOI] 10.1111/vcp.13311. [PMID] 38238980.
2024
Transcriptomic identification of genes associated with thrombosis and coagulation in lipopolysaccharide-exposed bovine monocyte-derived macrophages.
American journal of veterinary research. 85(12) [DOI] 10.2460/ajvr.24.04.0116. [PMID] 39348866.
2024
Use of RNA-seq to identify genes encoding cytokines and chemokines activated following uptake and processing a candidate peptide vaccine developed against Mycobacterium aviumsubsp. paratuberculosis
Brazilian Journal of Veterinary Medicine. 46 [DOI] 10.29374/2527-2179.bjvm002723. [PMID] 38282832.
2022
Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma
American Journal of Biomedical and Life Sciences. 9(1):84-96 [DOI] doi: 10.11648/j.ajbls.20210901.21.
2022
Salmon poisoning disease in dogs: clinical presentation, diagnosis and treatment.
Brazilian journal of veterinary medicine. 44 [DOI] 10.29374/2527-2179.bjvm004822. [PMID] 36475066.
2019
A peptide-based vaccine for Mycobacterium avium subspecies paratuberculosis.
Vaccine. 37(21):2783-2790 [DOI] 10.1016/j.vaccine.2019.04.040. [PMID] 31003915.
2018
A Mycobacterium avium subsp. paratuberculosis relA deletion mutant and a 35 kDa major membrane protein elicit development of cytotoxic T lymphocytes with ability to kill intracellular bacteria.
Veterinary research. 49(1) [DOI] 10.1186/s13567-018-0549-3. [PMID] 29941017.
2017
A nano particle vector comprised of poly lactic-co-glycolic acid and monophosphoryl lipid A and recombinant Mycobacterium avium subsp paratuberculosis peptides stimulate a pro-immune profile in bovine macrophages.
Journal of applied microbiology. 123(1):54-65 [DOI] 10.1111/jam.13491. [PMID] 28502107.
2016
In Vitro Evaluation of the Biological Responses of Canine Macrophages Challenged with PLGA Nanoparticles Containing Monophosphoryl Lipid A.
PloS one. 11(11) [DOI] 10.1371/journal.pone.0165477. [PMID] 27835636.
2015
Blocking the mitogen activated protein kinase-p38 pathway is associated with increase expression of nitric oxide synthase and higher production of nitric oxide by bovine macrophages infected with Mycobacterium avium subsp paratuberculosis.
Veterinary immunology and immunopathology. 164(1-2):1-9 [DOI] 10.1016/j.vetimm.2015.01.007. [PMID] 25700780.
2015
Mycobacterium avium Subspecies paratuberculosis Recombinant Proteins Modulate Antimycobacterial Functions of Bovine Macrophages.
PloS one. 10(6) [DOI] 10.1371/journal.pone.0128966. [PMID] 26076028.
2013
Mannosylated lipoarabinomannans from Mycobacterium avium subsp. paratuberculosis alters the inflammatory response by bovine macrophages and suppresses killing of Mycobacterium avium subsp. avium organisms.
PloS one. 8(9) [DOI] 10.1371/journal.pone.0075924. [PMID] 24098744.
2008
Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
Journal of leukocyte biology. 83(1):48-55 [PMID] 17913973.
2008
Effects of nuclear factor-kappaB on regulation of cytokine expression and apoptosis in bovine monocytes exposed to Mycobacterium avium subsp paratuberculosis.
American journal of veterinary research. 69(6):804-10 [DOI] 10.2460/ajvr.69.6.804. [PMID] 18518662.
2008
Review paper: modulation of mononuclear phagocyte function by Mycobacterium avium subsp. paratuberculosis.
Veterinary pathology. 45(6):829-41 [DOI] 10.1354/vp.45-6-829. [PMID] 18984786.
2008
Role of cell membrane receptors in the suppression of monocyte anti-microbial activity against Mycobacterium avium subsp. paratuberculosis.
Microbial pathogenesis. 44(3):215-23 [PMID] 18079089.
2007
Cell membrane receptors on bovine mononuclear phagocytes involved in phagocytosis of Mycobacterium avium subsp paratuberculosis.
American journal of veterinary research. 68(9):975-80 [PMID] 17764412.
2007
Role of the MAPK(ERK) pathway in regulation of cytokine expression by Mycobacterium avium subsp paratuberculosis-exposed bovine monocytes.
American journal of veterinary research. 68(6):625-30 [PMID] 17542695.
2007
Role of the mitogen-activated protein kinase pathway in the differential response of bovine monocytes to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
Microbes and infection. 9(14-15):1545-52 [PMID] 18035573.
2006
Mitogen activated protein kinase p38 pathway is an important component of the anti-inflammatory response in Mycobacterium avium subsp. paratuberculosis-infected bovine monocytes.
Microbial pathogenesis. 41(2-3):59-66 [PMID] 16716561.
2006
Mucosal immune response in cattle with subclinical Johne’s disease.
Veterinary pathology. 43(2):127-35 [PMID] 16537930.
2006
Regulation by Jun N-terminal kinase/stress activated protein kinase of cytokine expression in Mycobacterium avium subsp paratuberculosis-infected bovine monocytes.
American journal of veterinary research. 67(10):1760-5 [PMID] 17014329.
2005
A critical role of interleukin-10 in the response of bovine macrophages to infection by Mycobacterium avium subsp paratuberculosis.
American journal of veterinary research. 66(4):721-6 [PMID] 15900955.
2005
Expression of interleukin-10 and suppressor of cytokine signaling-3 associated with susceptibility of cattle to infection with Mycobacterium avium subsp paratuberculosis.
American journal of veterinary research. 66(7):1114-20 [PMID] 16111147.

Education

Residency in Veterinary Clinical Pathology
2008-2009 · University of Minnesota, College of Veterinary Medicine, St Paul, MN
Ph.D
2003-2008 · University of Minnesota, St Paul, MN
MS Immunology track
2001-2002 · Federal University of Santa Maria, RS, Brazil
Residency in Veterinary Clinical Pathology
2000-2001 · UNESP, Botucatu, SP, Brazil
Doctor of Veterinary Medicine
1994-1999 · Federal University of Parana, PR, Brazil

Contact Details

Phones:
Business:
(352) 294-4153
Emails:
Business:
cdesouza@ufl.edu
Addresses:
Business Street:
2015 SW 16TH AVE
GAINESVILLE FL 32608