John B Dame

John B Dame,

Emeritus Professor

Department: Department of Infectious Diseases & Immunology
Business Phone: (352) 294-4118
Business Email: damej@ufl.edu

Research Profile

Research interests of the Dame lab include: Emerging and re-emerging pathogens of animals and man; Molecular biology of the malaria parasite; Aspartic proteases as antimalarial drug targets; Vaccine antigen delivery for an improved malaria vaccine; Improved diagnosis of malaria infections; and the Genetic basis for adaptation of Plasmodium falciparum for sustained transmission in the New World.

Two studies are the current focus of the lab:

Efforts to control malaria in various locations are approaching pre-elimination. To succeed in malaria elimination, it is essential to be able to diagnose all infections. Asymptomatic, submicroscopic infections, and P. falciparum lines that have lost the HRP2 antigen biomarker, are detection resistant. Our lab utilizes a diagnostic test designed to detect an essential biomarker, which also has an ultralow level of detection (18S rRNA-qRT-PCR). Preparations are underway to utilize this approach combined with other assays to identify the proportion of malaria infections missed by current methods and estimate their potential contribution to sustained transmission in a low transmission setting. These quality controlled diagnostic services are available to public health laboratories requesting assistance for epidemiologic studies.

Progress is being made in our lab to characterize the evolution of P. falciparum on Hispaniola via whole genome sequencing. Following its introduction five hundred years ago from Africa, the ecosystems of the New World have applied powerful selective pressures on the parasite. Prominent among the selective pressures has been the requirement to utilize novel Anopheline mosquito vectors, as well as the opportunity to infect New World primates in some regions. The genetic basis underlying this successful adaptation is being characterized.

Open Researcher and Contributor ID (ORCID)

0000-0002-5772-5454

Publications

2020
Genetic Markers of Adaptation of Plasmodium falciparum to Transmission by American Vectors Identified in the Genomes of Parasites from Haiti and South America
mSphere. 5(5) [DOI] 10.1128/msphere.00937-20.
2020
Genetic Markers of Adaptation of Plasmodium falciparum to Transmission by American Vectors Identified in the Genomes of Parasites from Haiti and South America.
mSphere. 5(5) [DOI] 10.1128/mSphere.00937-20. [PMID] 33087522.
2018
Elimination or more accurate estimation? Investigation of trends in malaria diagnoses in the Ouest Department of Haiti from 2008 to 2017.
PloS one. 13(6) [DOI] 10.1371/journal.pone.0198070. [PMID] 29879170.
2017
Submicroscopic malaria infections in pregnant women from six departments in Haiti.
Tropical medicine & international health : TM & IH. 22(8):1030-1036 [DOI] 10.1111/tmi.12909. [PMID] 28609010.
2015
Enzymatic Characterization of Recombinant Food Vacuole Plasmepsin 4 from the Rodent Malaria Parasite Plasmodium berghei.
PloS one. 10(10) [DOI] 10.1371/journal.pone.0141758. [PMID] 26510189.
2015
High prevalence of asymptomatic malaria infections: a cross-sectional study in rural areas in six departments in Haiti.
Malaria journal. 14 [DOI] 10.1186/s12936-015-1051-2. [PMID] 26689195.
2015
Malaria elimination in Haiti by the year 2020: an achievable goal?
Malaria journal. 14 [DOI] 10.1186/s12936-015-0753-9. [PMID] 26043728.
2013
Survey of Plasmodium falciparum multidrug resistance-1 and chloroquine resistance transporter alleles in Haiti.
Malaria journal. 12 [DOI] 10.1186/1475-2875-12-426. [PMID] 24252305.
2011
Ehrlichia ruminantium in Amblyomma variegatum and domestic ruminants in the Caribbean.
Journal of medical entomology. 48(2):485-8 [PMID] 21485394.
View on: PubMed
2010
Apical surface expression of aspartic protease Plasmepsin 4, a potential transmission-blocking target of the plasmodium ookinete.
The Journal of biological chemistry. 285(11):8076-83 [DOI] 10.1074/jbc.M109.063388. [PMID] 20056606.
2010
Plasmepsin 4-deficient Plasmodium berghei are virulence attenuated and induce protective immunity against experimental malaria.
The American journal of pathology. 176(1):205-17 [DOI] 10.2353/ajpath.2010.090504. [PMID] 20019192.
2010
Rickettsia africae in Amblyomma variegatum and domestic ruminants on eight Caribbean islands.
The Journal of parasitology. 96(6):1086-8 [DOI] 10.1645/GE-2552.1. [PMID] 21158615.
2009
Recombinant plasmepsin 1 from the human malaria parasite plasmodium falciparum: enzymatic characterization, active site inhibitor design, and structural analysis.
Biochemistry. 48(19):4086-99 [DOI] 10.1021/bi802059r. [PMID] 19271776.
2009
Role of Plasmodium falciparum digestive vacuole plasmepsins in the specificity and antimalarial mode of action of cysteine and aspartic protease inhibitors.
Antimicrobial agents and chemotherapy. 53(12):4968-78 [DOI] 10.1128/AAC.00882-09. [PMID] 19752273.
2008
Clinical inquiries. Does antepartum perineal massage reduce intrapartum lacerations?
The Journal of family practice. 57(7):480-1 [PMID] 18625174.
View on: PubMed
2007
Analysis of binding interactions of pepsin inhibitor-3 to mammalian and malarial aspartic proteases.
Biochemistry. 46(49):14198-205 [PMID] 18004881.
View on: PubMed
2007
Critical roles for the digestive vacuole plasmepsins of Plasmodium falciparum in vacuolar function.
Molecular microbiology. 65(1):64-75 [PMID] 17581121.
View on: PubMed
2007
Effects on growth, hemoglobin metabolism and paralogous gene expression resulting from disruption of genes encoding the digestive vacuole plasmepsins of Plasmodium falciparum.
International journal for parasitology. 37(3-4):317-27 [PMID] 17207486.
View on: PubMed
2006
Structure of the aspartic protease plasmepsin 4 from the malarial parasite Plasmodium malariae bound to an allophenylnorstatine-based inhibitor.
Acta crystallographica. Section D, Biological crystallography. 62(Pt 3):246-52 [PMID] 16510971.
View on: PubMed
2005
Active-site specificity of digestive aspartic peptidases from the four species of Plasmodium that infect humans using chromogenic combinatorial peptide libraries.
Biochemistry. 44(6):1768-79 [PMID] 15697202.
View on: PubMed
2005
Crystallization and preliminary X-ray analysis of the aspartic protease plasmepsin 4 from the malarial parasite Plasmodium malariae.
Acta crystallographica. Section F, Structural biology and crystallization communications. 61(Pt 2):228-31 [PMID] 16511002.
View on: PubMed
2005
Expression and characterization of the ATP-binding domain of a malarial Plasmodium vivax gene homologous to the B-subunit of the bacterial topoisomerase DNA gyrase.
Molecular and biochemical parasitology. 140(1):107-17 [PMID] 15694492.
View on: PubMed
2004
Comparative structural analysis and kinetic properties of lactate dehydrogenases from the four species of human malarial parasites.
Biochemistry. 43(20):6219-29 [PMID] 15147206.
View on: PubMed
2004
Depletion of natural killer cells does not result in neurologic disease due to Sarcocystis neurona in mice with severe combined immunodeficiency.
The Journal of parasitology. 90(4):782-8 [PMID] 15357069.
View on: PubMed
2004
Genetic disruption of the Plasmodium falciparum digestive vacuole plasmepsins demonstrates their functional redundancy.
The Journal of biological chemistry. 279(52):54088-96 [PMID] 15491999.
View on: PubMed
2004
Infection of immunodeficient horses with Sarcocystis neurona does not result in neurologic disease.
Clinical and diagnostic laboratory immunology. 11(6):1134-9 [PMID] 15539518.
View on: PubMed
2004
Recombinant expression and enzymatic subsite characterization of plasmepsin 4 from the four Plasmodium species infecting man.
Molecular and biochemical parasitology. 135(1):101-9 [PMID] 15287591.
View on: PubMed
2003
Naturally occurring Sarcocystis infection in domestic cats (Felis catus).
International journal for parasitology. 33(8):877-83 [PMID] 12865087.
View on: PubMed
2003
Plasmepsin 4, the food vacuole aspartic proteinase found in all Plasmodium spp. infecting man.
Molecular and biochemical parasitology. 130(1):1-12 [PMID] 14550891.
View on: PubMed
2003
Structural insights into the activation of P. vivax plasmepsin.
Journal of molecular biology. 329(3):505-24 [PMID] 12767832.
View on: PubMed
2002
Molecular characterisation of a major 29 kDa surface antigen of Sarcocystis neurona.
International journal for parasitology. 32(2):217-25 [PMID] 11812499.
View on: PubMed
2002
Sarcocystis meningoencephalitis in a northern gannet (Morus bassanus).
Journal of wildlife diseases. 38(2):432-7 [PMID] 12038144.
View on: PubMed
2002
Sarcocystis neurona: parasitemia in a severe combined immunodeficient (SCID) horse fed sporocysts.
Experimental parasitology. 100(3):150-4 [PMID] 12173399.
View on: PubMed
2002
The effect of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) on granulocyte macrophage-colony stimulating factor (GM-CSF) production by neuronal precursor cells.
European cytokine network. 13(1):128-33 [PMID] 11956032.
View on: PubMed
2002
The shikimate pathway and its branches in apicomplexan parasites.
The Journal of infectious diseases. 185 Suppl 1:S25-36 [PMID] 11865437.
View on: PubMed
2001
Sporocyst size of isolates of Sarcocystis shed by the Virginia opossum (Didelphis virginiana).
Veterinary parasitology. 95(2-4):305-11 [PMID] 11223210.
View on: PubMed
2001
The nine-banded armadillo (Dasypus novemcinctus) is an intermediate host for Sarcocystis neurona.
International journal for parasitology. 31(4):330-5 [PMID] 11306111.
View on: PubMed
2001
The nine-banded armadillo (Dasypus novemcinctus) is naturally infected with Sarcocystis neurona.
International journal for parasitology. 31(4):325-9 [PMID] 11306110.
View on: PubMed
2001
The striped skunk (Mephitis mephitis) is an intermediate host for Sarcocystis neurona.
International journal for parasitology. 31(8):843-9 [PMID] 11403777.
View on: PubMed
2001
Viability of Sarcocystis neurona sporocysts and dose titration in gamma-interferon knockout mice.
Veterinary parasitology. 95(2-4):223-31 [PMID] 11223202.
View on: PubMed
1999
Karyotype and synteny among the chromosomes of all four species of human malaria parasite.
Molecular and biochemical parasitology. 101(1-2):23-32 [PMID] 10413040.
View on: PubMed
1996
Current status of the Plasmodium falciparum genome project.
Molecular and biochemical parasitology. 79(1):1-12 [PMID] 8844667.
View on: PubMed
1992
UNUSUAL POPULATION GENETICS OF A PARASITIC NEMATODE: mtDNA VARIATION WITHIN AND AMONG POPULATIONS.
Evolution; international journal of organic evolution. 46(2):470-476 [DOI] 10.1111/j.1558-5646.1992.tb02052.x. [PMID] 28564029.

Grants

Jul 2016 – Jun 2017
Eliminating Malaria from Haiti. Reinventing DNA to Eradicate Endemic Parasites
Role: Principal Investigator
Funding: FIREBIRD BIOMOLECULAR SCIENCES LLC via NATL INST OF HLTH NIAID

Patents

Issued September 2007
Detection of Sarcocystis Neurona
#7,273,709
Issued October 2004
Detection of Sarcocystis Neurona
#6,808,714

Education

Postdoctoral fellowship
1977-1980 · University of North Carolina, Chapel Hill
PhD in Biochemistry
1977 · University of Washington

Teaching Profile

Courses Taught
2006,2008-2010,2010-2014,2012-2018,2014-2018,2016-2018,2018
VME6464 Molecular Pathogenesis
2008,2012-2016,2016
VME6934 Topics in Veterinary Medical Sciences

Contact Details

Phones:
Business:
(352) 294-4118
Emails:
Business:
damej@ufl.edu

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