John A Bowden

John A Bowden

Assistant Professor

Department: Department of Physiological Sciences
Business Phone: (941) 716-4431
Business Email: john.bowden@ufl.edu

Accomplishments

Mentor Accolade
2017 · Material Measurement Laboratory (NIST)
Measurement Science Excellence Accolade
2016 · Material Measurement Laboratory (NIST)
Research Chemist
2013-2018 · Marine Biochemical Sciences Group, Chemical Sciences Division, National Institute of Standards and Technology
NIST/NRC Postdoctoral Fellow
2011-2013 · Environmental Chemical Sciences Group, Chemical Sciences Division, National Institute of Standards and Technology
Postdoctoral Research Fellowship/Associateship
2011 · NIST/NRC
Postdoctoral Fellow
2009-2011 · Department of Biochemistry and Molecular Biology, Saint Louis University
HHMI Science for Life Graduate Mentor Award
2009 · HHMI
Chemistry Department Teaching Award
2007 · University of Florida
Graduate Student Teaching Assistant Award
2006 · College of Liberal Arts and Sciences, University of Florida
Chemistry Department Teaching Award
2004 · University of Florida

Research Profile

The research I am pursuing in my laboratory focuses on employing mass spectrometric methods at the chem/bio interface, with a long-standing interest in endocrine disruption and environmental chemistry. A new drive in the laboratory is focused on studying the interplay between external measures of exposure (e.g., anthropogenic contaminants) and internal measures of exposure (e.g., lipids, hormones), in the context of health and disease. The tools we use to study these relationships are either gas or liquid chromatography and mass spectrometry (employing both targeted and non-targeted workflows). Beyond the implementation of these workflows, a considerable effort in the laboratory also focuses on improving key metrological aspects, including method development and optimization, quantitation, and quality control. Current work primarily employs workflows capable of performing lipidomics, metabolomics, and the measurement of chemicals of emerging concern (e.g., perfluorinated chemicals). Application areas are varied, encompassing environmental sampling, nutritional studies, lab-based models, and both wildlife and human health.

Publications

2021
A Review of Efforts to Improve Lipid Stability during Sample Preparation and Standardization Efforts to Ensure Accuracy in the Reporting of Lipid Measurements.
Lipids. 56(1):3-16 [DOI] 10.1002/lipd.12263. [PMID] 32519378.
2020
A rapid and simple method to quantify per- and polyfluoroalkyl substances (PFAS) in plasma and serum using 96-well plates.
MethodsX. 7 [DOI] 10.1016/j.mex.2020.101111. [PMID] 33134102.
2020
Author Correction: Developmental exposure of California mice to endocrine disrupting chemicals and potential effects on the microbiome-gut-brain axis at adulthood.
Scientific reports. 10(1) [DOI] 10.1038/s41598-020-69601-y. [PMID] 32694614.
2020
Concentrating Per- and Polyfluoroalkyl Substances (PFAS) in Municipal Solid Waste Landfill Leachate Using Foam Separation.
Environmental science & technology. 54(19):12550-12559 [DOI] 10.1021/acs.est.0c01266. [PMID] 32865409.
2020
Developmental exposure of California mice to endocrine disrupting chemicals and potential effects on the microbiome-gut-brain axis at adulthood.
Scientific reports. 10(1) [DOI] 10.1038/s41598-020-67709-9. [PMID] 32616744.
2020
Dissemination and analysis of the quality assurance (QA) and quality control (QC) practices of LC-MS based untargeted metabolomics practitioners.
Metabolomics : Official journal of the Metabolomic Society. 16(10) [DOI] 10.1007/s11306-020-01728-5. [PMID] 33044703.
2020
Ecotoxico-lipidomics: An emerging concept to understand chemical-metabolic relationships in comparative fish models.
Comparative biochemistry and physiology. Part D, Genomics & proteomics. 36 [DOI] 10.1016/j.cbd.2020.100742. [PMID] 32956922.
2020
Environmental lipidomics: understanding the response of organisms and ecosystems to a changing world.
Metabolomics : Official journal of the Metabolomic Society. 16(5) [DOI] 10.1007/s11306-020-01665-3. [PMID] 32307636.
2020
Estimating drug consumption during a college sporting event from wastewater using liquid chromatography mass spectrometry.
The Science of the total environment. 764 [DOI] 10.1016/j.scitotenv.2020.143963. [PMID] 33385644.
2020
Evaluation of extraction workflows for quantitative analysis of per- and polyfluoroalkyl substances: A case study using soil adjacent to a landfill.
The Science of the total environment. 760 [DOI] 10.1016/j.scitotenv.2020.143944. [PMID] 33341607.
2020
Examining maternal and environmental transfer of mercury into American alligator eggs.
Ecotoxicology and environmental safety. 189 [DOI] 10.1016/j.ecoenv.2019.110057. [PMID] 31835046.
2020
Lipid Annotator: Towards Accurate Annotation in Non-Targeted Liquid Chromatography High-Resolution Tandem Mass Spectrometry (LC-HRMS/MS) Lipidomics Using A Rapid and User-Friendly Software.
Metabolites. 10(3) [DOI] 10.3390/metabo10030101. [PMID] 32178227.
2020
Neurotoxicity assessment of triazole fungicides on mitochondrial oxidative respiration and lipids in differentiated human SH-SY5Y neuroblastoma cells.
Neurotoxicology. 80:76-86 [DOI] 10.1016/j.neuro.2020.06.009. [PMID] 32585290.
2020
Nontargeted lipidomics of novel human plasma reference materials: hypertriglyceridemic, diabetic, and African-American.
Analytical and bioanalytical chemistry. 412(27):7373-7380 [DOI] 10.1007/s00216-020-02910-3. [PMID] 32851459.
2020
Software Comparison for Nontargeted Analysis of PFAS in AFFF-Contaminated Soil.
Journal of the American Society for Mass Spectrometry. [DOI] 10.1021/jasms.0c00261. [PMID] 33226225.
2020
A histological evaluation of pansteatitis-affected Mozambique tilapia, Oreochromis mossambicus (Peters 1852), from different geographical locations in South Africa.
Journal of fish diseases. 43(10):1185-1199 [DOI] 10.1111/jfd.13184. [PMID] 32740987.
2020
Toward Comprehensive Per- and Polyfluoroalkyl Substances Annotation Using FluoroMatch Software and Intelligent High-Resolution Tandem Mass Spectrometry Acquisition.
Analytical chemistry. 92(16):11186-11194 [DOI] 10.1021/acs.analchem.0c01591. [PMID] 32806901.
2019
International Ring Trial of a High Resolution Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis.
Analytical chemistry. 91(22):14407-14416 [DOI] 10.1021/acs.analchem.9b02908. [PMID] 31638379.
2019
Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa.
Metabolomics : Official journal of the Metabolomic Society. 15(3) [DOI] 10.1007/s11306-019-1490-9. [PMID] 30838461.
2019
Per- and polyfluoroalkyl substances (PFAS) in plasma of the West Indian manatee (Trichechus manatus).
Marine pollution bulletin. 140:610-615 [DOI] 10.1016/j.marpolbul.2019.02.010. [PMID] 30803684.
2019
Perfluoroalkyl substances in diamondback terrapins (Malaclemys terrapin) in coastal South Carolina.
Chemosphere. 215:305-312 [DOI] 10.1016/j.chemosphere.2018.10.023. [PMID] 30321810.
2019
Software tool for internal standard based normalization of lipids, and effect of data-processing strategies on resulting values.
BMC bioinformatics. 20(1) [DOI] 10.1186/s12859-019-2803-8. [PMID] 31035918.
2019
Examining toxic trace element exposure in American alligators.
Environment international. 128:324-334 [DOI] 10.1016/j.envint.2019.04.035. [PMID] 31078001.
2018
Examining heat treatment for stabilization of the lipidome.
Bioanalysis. 10(5):291-305 [DOI] 10.4155/bio-2017-0209. [PMID] 29451398.
2018
Optimization of Folch, Bligh-Dyer, and Matyash sample-to-extraction solvent ratios for human plasma-based lipidomics studies.
Analytica chimica acta. 1037:351-357 [DOI] 10.1016/j.aca.2018.08.004. [PMID] 30292311.
2016
Effects of Crude Oil/Dispersant Mixture and Dispersant Components on PPARγ Activity in Vitro and in Vivo: Identification of Dioctyl Sodium Sulfosuccinate (DOSS; CAS #577-11-7) as a Probable Obesogen.
Environmental health perspectives. 124(1):112-9 [DOI] 10.1289/ehp.1409672. [PMID] 26135921.
2014
Influence of tissue, age, and environmental quality on DNA methylation in Alligator mississippiensis.
Reproduction (Cambridge, England). 147(4):503-13 [DOI] 10.1530/REP-13-0498. [PMID] 24398872.
2012
MALDI mass spectrometric imaging of cardiac tissue following myocardial infarction in a rat coronary artery ligation model.
Analytical chemistry. 84(2):1117-25 [DOI] 10.1021/ac202779h. [PMID] 22141424.
2009
Enhanced analysis of steroids by gas chromatography/mass spectrometry using microwave-accelerated derivatization.
Analytical chemistry. 81(16):6725-34 [DOI] 10.1021/ac900663c. [PMID] 19606839.
2009
Enhancement of chemical derivatization of steroids by gas chromatography/mass spectrometry (GC/MS).
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 877(27):3237-42 [DOI] 10.1016/j.jchromb.2009.08.005. [PMID] 19692297.
2009
Evaluation of derivatization strategies for the comprehensive analysis of endocrine disrupting compounds using GC/MS.
Journal of chromatographic science. 47(1):44-51 [PMID] 19161656.
View on: PubMed
2009
Investigation of HPLC-MS using a monolithic column to separate a diverse suite of steroids.
Journal of chromatographic science. 47(1):52-6 [PMID] 19161657.
View on: PubMed

Grants

Sep 2020 ACTIVE
Understanding Systemic Lupus Erythematosus Heterogeneity in a South Carolina Gullah African American Cohort using Exposomics
Role: Principal Investigator
Funding: US ARMY MED RES ACQUISITION
Aug 2020 ACTIVE
Vulnerable Coastal Communities Resilience to PFAS
Role: Co-Investigator
Funding: US ENVIRONMENTAL PRO AGCY
Jul 2020 ACTIVE
Hinkley Center Appropriation 2020-2021
Role: Project Manager
Funding: FL DEPT OF ENVIRONMENTAL PROTECT
Jun 2020 ACTIVE
2020 ASMS Postdoctoral Career Development Award – Juan Jose Aristizabal Henao
Role: Other
Funding: AM SOC FOR MASS SPECTROMETRY
May 2020 ACTIVE
Exploration of novel minimally-invasive sampling techniques for prospective marine mammal health assessments
Role: Principal Investigator
Funding: PADI FOU
Aug 2019 ACTIVE
The role of waste types on PFAS in landfills
Role: Co-Investigator
Funding: US ENVIRONMENTAL PRO AGCY

Education

Ph.D. in Analytical Chemistry
2008 · University of Florida
B.S. in Chemistry
2003 · University of Florida

Teaching Profile

Courses Taught
2020
CHM6165 Chemometrics
2019-2021
VME6932 Seminar in Physiological Sciences
2020-2021
VME6934 Topics in Veterinary Medical Sciences
2020-2021
VME4906 Problems in Veterinary Science
2019
VME5244 Physiology: Organ Systems
2021
CHM4910 Undergraduate Research

Contact Details

Phones:
Business:
(941) 716-4431
Emails:

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