Anthony F Barbet

Anthony F Barbet,

Professor Emeritus

Department: VM-DEAN
Business Phone: (352) 294-4115
Business Email: barbet@ufl.edu

Accomplishments

Research Professor Award
2007 · University of Florida
Research Excellence Award
2000 · Pfizer
Research Excellence Award
1993 · SmithKline-Beecham

Research Profile

Defining molecular mechanisms of pathogenesis in emerging infectious diseases, development of recombinant vaccines and improved molecular diagnostics. A long-term research focus has been to understand the molecular basis for persistent infections, beginning with investigations of antigenic variation in African trypanosomes and now including persistent infections caused by rickettsias of veterinary and human importance. An important finding has been the discovery of gene sequence mosaics in trypanosomes and Anaplasma. These mosaics are formed by contribution of sequence to genomic expression sites by different functional pseudogenes. This allows extensive surface diversity to be created from the small Anaplasma genomes because of the large number of different combinatorial possibilities. With the completion of genome sequence for these organisms, this research is now extending in two main directions: 1) analysis of gene expression and variation at the whole genome level. 2) development of transformation systems to knock-out virulence genes to develop new vaccines.

Publications

2021
Clinical and immunological responses in sheep after inoculation with Himar1-transformed Anaplasma phagocytophilum and subsequent challenge with a virulent strain of the bacterium.
Veterinary immunology and immunopathology. 231 [DOI] 10.1016/j.vetimm.2020.110165. [PMID] 33316536.
2021
Molecular characterization of “Candidatus Anaplasma testudinis”: An emerging pathogen in the threatened Florida gopher tortoise (Gopherus polyphemus).
Ticks and tick-borne diseases. 12(3) [DOI] 10.1016/j.ttbdis.2021.101672. [PMID] 33561680.
2020
Disruption of VirB6 Paralogs in Anaplasma phagocytophilum Attenuates Its Growth.
Journal of bacteriology. 202(23) [DOI] 10.1128/JB.00301-20. [PMID] 32928930.
2019
Assessing the clinical and bacteriological outcomes of vaccination with recombinant Asp14 and OmpA against A. phagocytophilum in sheep.
Veterinary immunology and immunopathology. 218 [DOI] 10.1016/j.vetimm.2019.109936. [PMID] 31590072.
2018
Quantifying known and emerging uterine pathogens, and evaluating their association with metritis and fever in dairy cows.
Theriogenology. 114:25-33 [DOI] 10.1016/j.theriogenology.2018.03.016. [PMID] 29574306.
2018
Viral Enrichment Methods Affect the Detection but Not Sequence Variation of West Nile Virus in Equine Brain Tissue.
Frontiers in veterinary science. 5 [DOI] 10.3389/fvets.2018.00318. [PMID] 30619900.
2018
VirB10 vaccination for protection against Anaplasma phagocytophilum.
BMC microbiology. 18(1) [DOI] 10.1186/s12866-018-1346-x. [PMID] 30563470.
2016
Development of a Quantitative PCR Assay for Differentiating the Agent of Heartwater Disease, Ehrlichia ruminantium, from the Panola Mountain Ehrlichia.
Transboundary and emerging diseases. 63(6):e260-e269 [DOI] 10.1111/tbed.12339. [PMID] 25807955.
2016
Persistent Infections and Immunity in Ruminants to Arthropod-Borne Bacteria in the Family Anaplasmataceae.
Annual review of animal biosciences. 4:177-97 [DOI] 10.1146/annurev-animal-022513-114206. [PMID] 26734888.
2016
Prevalence of Tick-Borne Pathogens in Host-Seeking Amblyomma americanum (Acari: Ixodidae) and Odocoileus virginianus (Artiodactyla: Cervidae) in Florida.
Journal of medical entomology. 53(4):949-956 [PMID] 27117680.
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2015
Complete Genome Sequence of Mycoplasma synoviae Strain WVU 1853T.
Genome announcements. 3(3) [DOI] 10.1128/genomeA.00563-15. [PMID] 26021934.
2015
Lambs immunized with an inactivated variant of Anaplasma phagocytophilum.
Acta veterinaria Scandinavica. 57 [DOI] 10.1186/s13028-015-0131-1. [PMID] 26205515.
2015
Reduced Infectivity in cattle for an outer membrane protein mutant of Anaplasma marginale.
Applied and environmental microbiology. 81(6):2206-14 [DOI] 10.1128/AEM.03241-14. [PMID] 25595772.
2014
Comparative Genomics Identifies a Potential Marker of Human-Virulent Anaplasma phagocytophilum.
Pathogens (Basel, Switzerland). 3(1):25-35 [DOI] 10.3390/pathogens3010025. [PMID] 25437606.
2014
Cultivation of Rickettsia amblyommii in tick cells, prevalence in Florida lone star ticks (Amblyomma americanum).
Parasites & vectors. 7 [DOI] 10.1186/1756-3305-7-270. [PMID] 24927809.
2014
Isolation of Tacaribe virus, a Caribbean arenavirus, from host-seeking Amblyomma americanum ticks in Florida.
PloS one. 9(12) [DOI] 10.1371/journal.pone.0115769. [PMID] 25536075.
2014
Knockout of an outer membrane protein operon of Anaplasma marginale by transposon mutagenesis.
BMC genomics. 15 [DOI] 10.1186/1471-2164-15-278. [PMID] 24725301.
2014
Point prevalence survey for tick-borne pathogens in military working dogs, shelter animals, and pet populations in northern Colombia.
Journal of special operations medicine : a peer reviewed journal for SOF medical professionals. 14(4):81-5 [PMID] 25399372.
View on: PubMed
2013
An emerging tick-borne disease of humans is caused by a subset of strains with conserved genome structure.
Pathogens (Basel, Switzerland). 2(3):544-55 [DOI] 10.3390/pathogens2030544. [PMID] 25437207.
2013
Surveillance for Tick-Borne Pathogens in Military Working Dogs, Shelter Animals and Pet Populations in Colombia
Journal of Veterinary Internal Medicine. 27:722-723
2012
Antigen variability in Anaplasma phagocytophilum during chronic infection of a reservoir host.
Microbiology (Reading, England). 158(Pt 10):2632-2641 [DOI] 10.1099/mic.0.059808-0. [PMID] 22859615.
2012
Determining the repertoire of immunodominant proteins via whole-genome amplification of intracellular pathogens.
PloS one. 7(4) [DOI] 10.1371/journal.pone.0036456. [PMID] 22558468.
2012
Evolution of antigen variation in the tick-borne pathogen Anaplasma phagocytophilum.
Molecular biology and evolution. 29(1):391-400 [DOI] 10.1093/molbev/msr229. [PMID] 21965342.
2012
Genome annotation of five Mycoplasma canis strains.
Journal of bacteriology. 194(15):4138-9 [DOI] 10.1128/JB.00664-12. [PMID] 22815452.
2012
Structure of the type IV secretion system in different strains of Anaplasma phagocytophilum.
BMC genomics. 13 [DOI] 10.1186/1471-2164-13-678. [PMID] 23190684.
2011
Investigation of endothelial cells as an in vivo nidus of Anaplasma marginale infection in cattle.
Veterinary microbiology. 153(3-4):264-73 [DOI] 10.1016/j.vetmic.2011.05.035. [PMID] 21680112.
2011
Multistrain genome analysis identifies candidate vaccine antigens of Anaplasma marginale.
Vaccine. 29(31):4923-32 [DOI] 10.1016/j.vaccine.2011.04.131. [PMID] 21596083.
2010
Transformation of Anaplasma marginale.
Veterinary parasitology. 167(2-4):167-74 [DOI] 10.1016/j.vetpar.2009.09.018. [PMID] 19837516.
2010
Variant-specific and diminishing immune responses towards the highly variable MSP2(P44) outer membrane protein of Anaplasma phagocytophilum during persistent infection in lambs.
Veterinary immunology and immunopathology. 133(2-4):117-24 [DOI] 10.1016/j.vetimm.2009.07.009. [PMID] 19695712.
2009
Antigen diversity in the parasitic bacterium Anaplasma phagocytophilum arises from selectively-represented, spatially clustered functional pseudogenes.
PloS one. 4(12) [DOI] 10.1371/journal.pone.0008265. [PMID] 20016821.
2009
Distinct ecologically relevant strains of Anaplasma phagocytophilum.
Emerging infectious diseases. 15(5):842-3 [DOI] 10.3201/eid1505.081502. [PMID] 19402993.
2009
Diversity of Anaplasma phagocytophilum strains, USA.
Emerging infectious diseases. 15(6):928-31 [DOI] 10.3201/eid1506.081610. [PMID] 19523294.
2009
Diversity of Ehrlichia ruminantium major antigenic protein 1-2 in field isolates and infected sheep.
Infection and immunity. 77(6):2304-10 [DOI] 10.1128/IAI.01409-08. [PMID] 19307215.
2009
Persistence mechanisms in tick-borne diseases.
The Onderstepoort journal of veterinary research. 76(1):53-8 [PMID] 19967928.
View on: PubMed
2009
varDB: common ground for a shifting landscape.
Trends in parasitology. 25(6):249-52 [DOI] 10.1016/j.pt.2009.03.003. [PMID] 19423393.
2008
In situ detection of Anaplasma spp. by DNA target-primed rolling-circle amplification of a padlock probe and intracellular colocalization with immunofluorescently labeled host cell von Willebrand factor.
Journal of clinical microbiology. 46(7):2314-9 [DOI] 10.1128/JCM.02197-07. [PMID] 18495855.
2008
Outer membrane protein sequence variation in lambs experimentally infected with Anaplasma phagocytophilum.
Infection and immunity. 76(1):120-6 [PMID] 17967854.
View on: PubMed
2007
Characterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale.
Clinical and vaccine immunology : CVI. 14(3):262-8 [PMID] 17215333.
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2007
In vivo endothelial cell infection by Anaplasma marginale.
Veterinary pathology. 44(1):116-8 [PMID] 17197636.
View on: PubMed
2006
Cloning and expression of the gene encoding the major surface protein 5 (MSP5) of Anaplasma phagocytophilum and potential application for serodiagnosis.
Veterinary clinical pathology. 35(4):418-25 [PMID] 17123248.
View on: PubMed
2006
Evaluation of E. ruminantium genes in DBA/2 mice as potential DNA vaccine candidates for control of heartwater.
Annals of the New York Academy of Sciences. 1078:424-37 [PMID] 17114751.
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2006
Structure of the expression site reveals global diversity in MSP2 (P44) variants in Anaplasma phagocytophilum.
Infection and immunity. 74(11):6429-37 [PMID] 16966408.
View on: PubMed
2006
Transformation of Anaplasma phagocytophilum.
BMC biotechnology. 6 [PMID] 17076894.
View on: PubMed
2005
DNA vaccination against respiratory syncytial virus in young calves.
Vaccine. 23(10):1242-50 [PMID] 15652666.
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2005
Identification of functional promoters in the msp2 expression loci of Anaplasma marginale and Anaplasma phagocytophilum.
Gene. 353(1):89-97 [PMID] 15935572.
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2004
Adhesion of outer membrane proteins containing tandem repeats of Anaplasma and Ehrlichia species (Rickettsiales: Anaplasmataceae) to tick cells.
Veterinary microbiology. 98(3-4):313-22 [PMID] 15036540.
View on: PubMed
2004
CD4+ T lymphocytes from Anaplasma marginale major surface protein 2 (MSP2) vaccinees recognize naturally processed epitopes conserved in MSP3.
Infection and immunity. 72(6):3688-92 [PMID] 15155686.
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2004
Characterization of the Anaplasma marginale msp2 locus and its synteny with the omp1/p30 loci of Ehrlichia chaffeensis and E. canis.
Gene. 325:115-21 [PMID] 14697516.
View on: PubMed
2003
Characterization of the major antigenic protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and its application for serodiagnosis of ehrlichiosis.
Clinical and diagnostic laboratory immunology. 10(4):520-4 [PMID] 12853379.
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2003
Conservation of a gene conversion mechanism in two distantly related paralogues of Anaplasma marginale.
Molecular microbiology. 47(3):633-43 [PMID] 12535066.
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2003
Development of improved vaccines for heartwater.
Developments in biologicals. 114:137-45 [PMID] 14677684.
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2003
Expression of multiple outer membrane protein sequence variants from a single genomic locus of Anaplasma phagocytophilum.
Infection and immunity. 71(4):1706-18 [PMID] 12654783.
View on: PubMed
2003
Simultaneous variation of the immunodominant outer membrane proteins, MSP2 and MSP3, during anaplasma marginale persistence in vivo.
Infection and immunity. 71(11):6627-32 [PMID] 14573687.
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2002
Antigenic variation of Anaplasma marginale msp2 occurs by combinatorial gene conversion.
Molecular microbiology. 43(5):1151-9 [PMID] 11918803.
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2002
Comparison of serological detection methods for diagnosis of Ehrlichia canis infections in dogs.
Journal of clinical microbiology. 40(9):3506-8 [PMID] 12202605.
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2002
Conservation of major surface protein 1 genes of Anaplasma marginale during cyclic transmission between ticks and cattle.
Gene. 282(1-2):95-102 [PMID] 11814681.
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2002
DNA vaccination with map1 gene followed by protein boost augments protection against challenge with Cowdria ruminantium, the agent of heartwater.
Vaccine. 20(7-8):1215-25 [PMID] 11803084.
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2002
Expression of Anaplasma marginale major surface protein 2 operon-associated proteins during mammalian and arthropod infection.
Infection and immunity. 70(11):6005-12 [PMID] 12379676.
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2002
Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent.
Veterinary immunology and immunopathology. 85(1-2):23-32 [PMID] 11867164.
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2001
A subset of Cowdria ruminantium genes important for immune recognition and protection.
Gene. 275(2):287-98 [PMID] 11587856.
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2001
Antibody responses to MAP 1B and other Cowdria ruminantium antigens are down regulated in cattle challenged with tick-transmitted heartwater.
Clinical and diagnostic laboratory immunology. 8(2):388-96 [PMID] 11238227.
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2001
Antigenic variation of Anaplasma marginale: major surface protein 2 diversity during cyclic transmission between ticks and cattle.
Infection and immunity. 69(5):3057-66 [PMID] 11292724.
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2001
Ingenious gene generation.
Trends in microbiology. 9(8):353-5; discussion 355 [PMID] 11514195.
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2001
Recombinant major antigenic protein 2 of Ehrlichia canis: a potential diagnostic tool.
Journal of clinical microbiology. 39(7):2494-9 [PMID] 11427559.
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2001
Reduction in mortality from heartwater in cattle, sheep and goats exposed to field challenge using an inactivated vaccine.
Veterinary parasitology. 97(4):295-308 [PMID] 11390083.
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2001
Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‘HGE agent’ as subjective synonyms of Ehrlichia phagocytophila.
International journal of systematic and evolutionary microbiology. 51(Pt 6):2145-2165 [DOI] 10.1099/00207713-51-6-2145. [PMID] 11760958.
2000
A polarized Th1 type immune response to Cowdria ruminantium infection is detected in immune DBA/2 mice.
The Journal of parasitology. 86(5):983-92 [PMID] 11128522.
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2000
Anaplasmosis control. Past, present, and future.
Annals of the New York Academy of Sciences. 916:501-9 [PMID] 11193665.
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2000
Antigenic variation of Anaplasma marginale by expression of MSP2 mosaics.
Infection and immunity. 68(11):6133-8 [PMID] 11035716.
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2000
CD8(+) T cell knockout mice are less susceptible to Cowdria ruminantium infection than athymic, CD4(+) T cell knockout, and normal C57BL/6 mice.
Veterinary parasitology. 93(2):159-72 [PMID] 11035234.
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2000
Comparison of efficacy of American and African Amblyomma ticks as vectors of heartwater (Cowdria ruminantium) infection by molecular analyses and transmission trials.
The Journal of parasitology. 86(1):44-9 [PMID] 10701562.
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2000
Detection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks.
Journal of clinical microbiology. 38(4):1539-44 [PMID] 10747140.
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2000
Establishment and characterization of an Oklahoma isolate of Anaplasma marginale in cultured Ixodes scapularis cells.
Veterinary parasitology. 87(4):301-13 [PMID] 10669100.
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2000
Expression of a gene encoding the major antigenic protein 2 homolog of ehrlichia chaffeensis and potential application for serodiagnosis.
Journal of clinical microbiology. 38(10):3705-9 [PMID] 11015387.
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2000
Sequence and expression analysis of a surface antigen gene family of the rickettsia Anaplasma marginale.
Gene. 253(1):45-53 [PMID] 10925201.
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1999
Comparison of surface proteins of Anaplasma marginale grown in tick cell culture, tick salivary glands, and cattle.
Infection and immunity. 67(1):102-7 [PMID] 9864202.
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1999
Potential value of major antigenic protein 2 for serological diagnosis of heartwater and related ehrlichial infections.
Clinical and diagnostic laboratory immunology. 6(2):209-15 [PMID] 10066656.
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1999
Prevalence of Cowdria ruminantium infection in Amblyomma hebraeum ticks from heartwater-endemic areas of Zimbabwe.
Epidemiology and infection. 123(2):309-16 [PMID] 10579452.
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1999
The map1 gene of Cowdria ruminantium is a member of a multigene family containing both conserved and variable genes.
Biochemical and biophysical research communications. 257(2):300-5 [PMID] 10198207.
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1999
Western immunoblot analysis of the antigens of Haemobartonella felis with sera from experimentally infected cats.
Journal of clinical microbiology. 37(5):1474-9 [PMID] 10203508.
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1998
A DNA vaccine protects mice against the rickettsial agent Cowdria ruminantium.
Parasite immunology. 20(3):111-9 [PMID] 9568614.
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1998
Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium.
Annals of the New York Academy of Sciences. 849:155-60 [PMID] 9668461.
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1998
Major antigenic proteins 1 and 2 of Cowdria ruminantium are targets for T-lymphocyte responses of immune cattle.
Annals of the New York Academy of Sciences. 849:372-4 [PMID] 9668489.
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1998
Molecular characterization of a 28 kDa surface antigen gene family of the tribe Ehrlichiae.
Biochemical and biophysical research communications. 247(3):636-43 [PMID] 9647746.
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1998
The inactivated Cowdria ruminantium vaccine for heartwater protects against heterologous strains and against laboratory and field tick challenge.
Vaccine. 16(11-12):1203-11 [PMID] 9682380.
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1996
A recombinant antigen from the heartwater agent (Cowdria ruminatium) reactive with antibodies in some southeastern United States white-tailed deer (Odocoileus virginianus), but not cattle, sera.
Journal of wildlife diseases. 32(3):424-30 [PMID] 8827667.
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1996
Evaluation of Anaplasma marginale major surface protein 3 (MSP3) as a diagnostic test antigen.
Journal of clinical microbiology. 34(2):270-76 [PMID] 8788999.
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1996
Expression of major surface protein 2 antigenic variants during acute Anaplasma marginale rickettsemia.
Infection and immunity. 64(3):836-41 [PMID] 8641789.
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1996
Sequence heterogeneity of the major antigenic protein 1 genes from Cowdria ruminantium isolates from different geographical areas.
Clinical and diagnostic laboratory immunology. 3(4):417-22 [PMID] 8807206.
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1995
Babesia bigemina: host factors affecting the invasion of erythrocytes.
Experimental parasitology. 80(1):76-84 [PMID] 7821413.
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1995
Development and evaluation of PCR assay for detection of low levels of Cowdria ruminantium infection in Amblyomma ticks not detected by DNA probe.
Journal of clinical microbiology. 33(1):166-72 [PMID] 7699036.
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1995
Immunisation of sheep against heartwater with inactivated Cowdria ruminantium.
Research in veterinary science. 58(1):46-9 [PMID] 7709059.
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1995
Laboratory reared Amblyomma hebraeum and Amblyomma variegatum ticks differ in their susceptibility to infection with Cowdria ruminantium.
Epidemiology and infection. 115(2):345-53 [PMID] 7589273.
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1995
Recent developments in the molecular biology of anaplasmosis.
Veterinary parasitology. 57(1-3):43-9 [PMID] 7597790.
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1994
Molecular cloning of a gene encoding the immunogenic 21 kDa protein of Cowdria ruminantium.
Microbiology (Reading, England). 140 ( Pt 8):2135-42 [PMID] 7921263.
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1994
Putative adhesins of Anaplasma marginale: major surface polypeptides 1a and 1b.
Infection and immunity. 62(10):4594-601 [PMID] 7927726.
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1994
Recombinant vaccinia virus expression of Anaplasma marginale surface protein MSP-1a: effect of promoters, leader sequences and GPI anchor sequence on antibody response.
Vaccine. 12(5):465-71 [PMID] 8023555.
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1994
Size variation of the major immunodominant protein of Cowdria ruminantium.
Clinical and diagnostic laboratory immunology. 1(6):744-6 [PMID] 8556531.
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1994
The immunoprotective Anaplasma marginale major surface protein 2 is encoded by a polymorphic multigene family.
Infection and immunity. 62(9):3808-16 [PMID] 8063397.
View on: PubMed
1993
An immunoblotting diagnostic assay for heartwater based on the immunodominant 32-kilodalton protein of Cowdria ruminantium detects false positives in field sera.
Journal of clinical microbiology. 31(10):2729-37 [PMID] 8253974.
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1993
Analysis of the Anaplasma marginale genome by pulsed-field electrophoresis.
Journal of general microbiology. 139(10):2439-44 [PMID] 7902862.
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1993
Derivation of the complete msp4 gene sequence of Anaplasma marginale without cloning.
Gene. 136(1-2):291-4 [PMID] 8294020.
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1993
Detection of Cowdria ruminantium by means of a DNA probe, pCS20 in infected bont ticks, Amblyomma hebraeum, the major vector of heartwater in southern Africa.
Epidemiology and infection. 110(1):95-104 [PMID] 8432329.
View on: PubMed
1993
Expression and immune recognition of the conserved MSP4 outer membrane protein of Anaplasma marginale.
Infection and immunity. 61(12):5245-51 [PMID] 7693596.
View on: PubMed
1993
The development of antibody to Cowdria ruminantium in mice and its role in heartwater disease.
Revue d'elevage et de medecine veterinaire des pays tropicaux. 46(1-2):197-201 [PMID] 8134632.
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1993
The importance of mosaic genes to trypanosome survival.
Parasitology today (Personal ed.). 9(2):63-6 [PMID] 15463711.
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1992
A cloned DNA probe for Cowdria ruminantium hybridizes with eight heartwater strains and detects infected sheep.
Journal of clinical microbiology. 30(4):981-6 [PMID] 1572987.
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1992
Surface epitope variation via mosaic gene formation is potential key to long-term survival of Trypanosoma brucei.
Molecular and biochemical parasitology. 53(1-2):33-44 [PMID] 1380125.
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1991
A cloned DNA probe identifies Cowdria ruminantium in Amblyomma variegatum ticks.
Journal of clinical microbiology. 29(11):2571-7 [PMID] 1774264.
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1991
The msp1 beta multigene family of Anaplasma marginale: nucleotide sequence analysis of an expressed copy.
Infection and immunity. 59(3):971-6 [PMID] 1671779.
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1991
Trypanosoma brucei: peptide mapping of partially homologous variable surface glycoproteins.
International journal for parasitology. 21(5):573-8 [PMID] 1743853.
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1990
Characterization of a repetitive DNA probe for Babesia bigemina.
Veterinary parasitology. 36(1-2):11-20 [PMID] 2382379.
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1990
Molecular basis for surface antigen size polymorphisms and conservation of a neutralization-sensitive epitope in Anaplasma marginale.
Proceedings of the National Academy of Sciences of the United States of America. 87(8):3220-4 [PMID] 1691504.
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1989
Detection and quantitation of Anaplasma marginale in carrier cattle by using a nucleic acid probe.
Journal of clinical microbiology. 27(2):279-84 [PMID] 2915021.
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1989
Immunization of cattle with the MSP-1 surface protein complex induces protection against a structurally variant Anaplasma marginale isolate.
Infection and immunity. 57(11):3666-9 [PMID] 2807542.
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1989
Shared surface epitopes among trypanosomes of the same serodeme expressing different variable surface glycoprotein genes.
Molecular and biochemical parasitology. 32(2-3):191-9 [PMID] 2467204.
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1989
Vaccines for parasitic infections.
Advances in veterinary science and comparative medicine. 33:345-75 [PMID] 2648776.
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1988
Detection of Anaplasma-marginale-infected tick vectors by using a cloned DNA probe.
Proceedings of the National Academy of Sciences of the United States of America. 85(3):919-23 [PMID] 3422471.
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1988
Immunization of cattle with a 36-kilodalton surface protein induces protection against homologous and heterologous Anaplasma marginale challenge.
Infection and immunity. 56(6):1526-31 [PMID] 3372018.
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1988
Molecular size variations in an immunoprotective protein complex among isolates of Anaplasma marginale.
Infection and immunity. 56(6):1567-73 [PMID] 3372021.
View on: PubMed
1988
Recognition of conserved surface protein epitopes on Anaplasma centrale and Anaplasma marginale isolates from Israel, Kenya and the United States.
International journal for parasitology. 18(1):33-8 [PMID] 2452797.
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1987
Antigenic specificity of convalescent serum from cattle with haemophilus somnus-induced experimental abortion.
Infection and immunity. 55(6):1381-6 [PMID] 3570470.
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1987
Characterization of a neutralization-sensitive epitope on the Am 105 surface protein of Anaplasma marginale.
International journal for parasitology. 17(7):1279-85 [PMID] 2448258.
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1987
Characterization of an immunoprotective protein complex of Anaplasma marginale by cloning and expression of the gene coding for polypeptide Am105L.
Infection and immunity. 55(10):2428-35 [PMID] 2443451.
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1987
Structural features of antigenic determinants on variant surface glycoproteins from Trypanosoma brucei.
Molecular immunology. 24(7):707-13 [PMID] 2443842.
View on: PubMed
1986
Detection of an Anaplasma marginale common surface protein present in all stages of infection.
Journal of clinical microbiology. 23(6):1078-83 [PMID] 3711298.
View on: PubMed
1986
Immunization with an isolate-common surface protein protects cattle against anaplasmosis.
Science (New York, N.Y.). 231(4743):1299-302 [PMID] 3945825.
View on: PubMed
1986
Trypanosoma brucei: a fluorescence and spin label study of the membranes of the bloodstream form.
Experimental parasitology. 62(3):423-9 [PMID] 3780935.
View on: PubMed
1985
Conservation of structure detected in two trypanosome surface glycoproteins by amino acid sequence alignment.
Molecular and biochemical parasitology. 14(2):175-85 [PMID] 3838798.
View on: PubMed
1985
Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines.
The Journal of general virology. 66 ( Pt 5):1139-43 [PMID] 3923155.
View on: PubMed
1985
Presence of common antigens, including major surface protein epitopes, between the cattle (intraerythrocytic) and tick stages of Anaplasma marginale.
Infection and immunity. 50(3):881-6 [PMID] 2415457.
View on: PubMed
1984
Amino terminal sequence homology among variant surface glycoproteins of African trypanosomes.
Molecular and biochemical parasitology. 12(3):287-98 [PMID] 6482907.
View on: PubMed
1983
Comparison of proteins synthesized by two different isolates of Anaplasma marginale.
Infection and immunity. 40(3):1068-74 [PMID] 6852911.
View on: PubMed
1983
Preferential immune response to virion surface glycoproteins by caprine arthritis-encephalitis virus-infected goats.
Infection and immunity. 41(2):657-65 [PMID] 6307878.
View on: PubMed
1982
Cross-neutralization of two different trypanosome populations derived from a single organism.
Nature. 300(5891):453-6 [PMID] 6183596.
View on: PubMed
1982
Identification and analysis of parasite surface antigens and parasite-induced antigens on host cells.
Veterinary parasitology. 10(2-3):181-9 [PMID] 6753313.
View on: PubMed
1982
Immune depression in African trypanosomiasis: the role of antigenic competition.
Clinical and experimental immunology. 47(2):234-42 [PMID] 7075022.
View on: PubMed
1982
Purification of Trypanosoma brucei variable surface glycoproteins: analysis of degradation occurring during isolation.
Parasitology. 85 (Pt 3):511-22 [PMID] 7177702.
View on: PubMed
1981
Isotypic surface glycoproteins of trypanosomes.
Immunology. 44(2):223-32 [PMID] 6170573.
View on: PubMed
1980
Trypanosoma brucei: radioimmunoassay of variant surface glycoproteins from organisms grown in vitro and in vivo.
Experimental parasitology. 50(2):233-9 [PMID] 6157559.
View on: PubMed
1979
Evidence for reappearance of Trypanosoma brucei variable antigen types in relapse populations.
The Journal of parasitology. 65(5):673-9 [PMID] 92560.
View on: PubMed
1978
Crossreacting determinants in variant-specific surface antigens of African trypanosomes.
Proceedings of the National Academy of Sciences of the United States of America. 75(4):1989-93 [PMID] 77021.
View on: PubMed
1977
Activation of complement by variant-specific surface antigen of trypanosoma brucei.
Nature. 270(5636):438-40 [PMID] 593363.
View on: PubMed

Grants

Jul 2014 – Jun 2016
Structure and function of VirB6 in the Rickettsiales
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAID

Patents

Issued December 2007
Polynucleotides and Polypeptides of Anaplasma Phagocytophilum and Methods of Using the Same
#7,304,139
Issued November 2003
Nucleic Acid Vaccines Against Rickettsial Diseases And Methods Of Use
#6,653,128
Published November 2003
Nucleic Acid Vaccines Against Rickettsial Diseases and Methods of Use
#US-2004-0126871-A1
Issued July 2003
Nucleic Acid Vaccines Against Rickettsial Diseases And Methods Of Use
#6,593,147
Published February 2002
Ehrlichia Ruminantium Polypeptides, Antigens, Polynucleotides and Methods of Use
#US-2003-0044422-A1
Issued January 2002
MATERIALS AND METHODS FOR PREVENTION OR REDUCTION OF THE SEVERITY OF HEARTWATER
#6,342,230
Issued June 2001
Nucleic Acid Vaccines Against Rickettsial Diseases and Methods of Use
#6,251,872
Issued February 2000
Nucleic Acid Vaccines Against Rickettsial Diseases of Animals and Humans
#6,025,338

Education

PhD – Biochemical Parasitology
1977 · University of Cambridge

Teaching Profile

Courses Taught
2006,2008-2010,2010-2014,2012-2016,2014-2016
VME6464 Molecular Pathogenesis
2006,2008-2010,2010-2016,2012-2016,2016
VME6934 Topics in Veterinary Medical Sciences
2015
VEM5131 Vet Molecular Biology

Contact Details

Phones:
Business:
(352) 294-4115
Emails:
Business:
barbet@ufl.edu

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