Patrick Thompson
352-278-5200
Equipment:
- Illumina Miseq
- Molecular Biology
- Gel electrophoresis
- Thermocyclers
- PCR hoods
- Biosafety cabinets
- Microcentrifuges (refrigerated & non
- Real time PCR (FAST) machine
- Incubator (non CO2 and CO2)
- Centrifuge (3000 xg max)
- Vacuum centrifuge
- Ultracentrifuge
- Clinical pathology – hematology sample preparation
Training or skills
- DNA and RNA extraction methodologies
- PCR (Polymerase Chain Reaction) techniques
- Real Time PCR methods to quantify viral RNA/DNA levels in extracted samples
- Gel Electrophoresis
- Purification of nucleic acid fragments from the gel
- Primer design to create new primers used in all PCR methods
- Consensus PCR methods used to determine the presence of a virus belonging to a larger category of viruses such as an order, family or genus
- Next generation library prep
- Cell culture – mammalian and fish cell lines
- RT PCR / Quantitative RT PCR
- Animal skills: dog/cat/mouse handling, phlebotomy
- Archiving of biological samples following NIST protocols