Patrick Thompson

thompsonp@ufl.edu  

352-278-5200

Equipment:

  • Illumina Miseq
  • Molecular Biology
  • Gel electrophoresis
  • Thermocyclers
  • PCR hoods
  • Biosafety cabinets
  • Microcentrifuges (refrigerated & non
  • Real time PCR (FAST) machine
  • Incubator (non CO2 and CO2)
  • Centrifuge (3000 xg max)
  • Vacuum centrifuge
  • Ultracentrifuge
  • Clinical pathology – hematology sample preparation

Training or skills

  • DNA and RNA extraction methodologies
  • PCR (Polymerase Chain Reaction) techniques
  • Real Time PCR methods to quantify viral RNA/DNA levels in extracted samples
  • Gel Electrophoresis
  • Purification of nucleic acid fragments from the gel
  • Primer design to create new primers used in all PCR methods
  • Consensus PCR methods used to determine the presence of a virus belonging to a larger category of viruses such as an order, family or genus
  • Next generation library prep
  • Cell culture – mammalian and fish cell lines
  • RT PCR / Quantitative RT PCR
  • Animal skills: dog/cat/mouse handling, phlebotomy
  • Archiving of biological samples following NIST protocols