Kim Ahrens

Supervisor – Dr.┬áMarsella

ahrensk@ufl.edu

Equipment

  • Elisa reader Dynex (visible light)
  • Dual head microscope
  • microscope with old nikon coolpix camera
  • Delphin Trans epidermal water loss reader
  • PCR machine used for RT reactions only
  • 9600 PCR machine
  • StepOne Plus AB Biosystem Real time PCR machine
  • Nuwind refrigerated centrifuge
  • Harrier refrigerated centrifruge
  • minus 80 freezer
  • Amerex shaking water bath
  • Analytical balance
  • 2 steel kennels-multiple med dog travel kennels
  • Old style projector for slides
  • Lab Vision Autostainer

Training or Skills

  • Cell culturing of keratinocytes, and isolation of canine and human lymphocytes and culturing for studies
  • Cytokine ELISA testing
  • Immunohistochemistry and ImageJ program NIH
  • Immunofluorescent staining of slides
  • Licensed Histologist in previous life
  • Extraction of RNA and DNA from fresh, ethanol fixed and paraffin tissue/cells
  • Quantitative PCR (licensed technologist in molecular biology)
  • Cloning
  • Primer design and PCR test development
  • Flow cytometry
  • Southern and western blots
  • Animal handling skills dogs, cows and horses

University of Florida

IFAS

As part of both the Institute of Food and Agricultural Sciences and UF Health, Veterinary Medicine is dedicated to advancing animal, human and environmental health through teaching, research, extension and patient care.

IFAS

UNIVERSITY OF FLORIDA

Animal Hospitals

Need animal care? Visit the UF Small Animal and Large Animal Hospitals. From dogs, cats, birds and exotics to horses, cattle, llamas, pigs and many other large farm or food animals, our experienced veterinarian staff is ready to assist.

Animal Airwaves

Syndicated Podcast

Animal Airwaves

Animal Airwaves is a daily radio series that features one-minute segments relating to animal health, ranging from insights into animal behavior to veterinary patient care breakthroughs and trends.

Animal Airwaves