Supervisor – Dr. Marsella
ahrensk@ufl.edu
Equipment
- Elisa reader Dynex (visible light)
- Dual head microscope
- microscope with old nikon coolpix camera
- Delphin Trans epidermal water loss reader
- PCR machine used for RT reactions only
- 9600 PCR machine
- StepOne Plus AB Biosystem Real time PCR machine
- Nuwind refrigerated centrifuge
- Harrier refrigerated centrifruge
- minus 80 freezer
- Amerex shaking water bath
- Analytical balance
- 2 steel kennels-multiple med dog travel kennels
- Old style projector for slides
- Lab Vision Autostainer
Training or Skills
- Cell culturing of keratinocytes, and isolation of canine and human lymphocytes and culturing for studies
- Cytokine ELISA testing
- Immunohistochemistry and ImageJ program NIH
- Immunofluorescent staining of slides
- Licensed Histologist in previous life
- Extraction of RNA and DNA from fresh, ethanol fixed and paraffin tissue/cells
- Quantitative PCR (licensed technologist in molecular biology)
- Cloning
- Primer design and PCR test development
- Flow cytometry
- Southern and western blots
- Animal handling skills dogs, cows and horses
