becarbia@ufl.edu
Equipment
- CO2 Incubator
- BioRad Chemi/UV transluminator
- Centrifuges, mini
- Dental cleaning kit and toolsPCR machine used for RT reactions only
- Dremel tool kit
- Electronic/analytical balance
- Hot plates, stirrers and shakers
- Ophthalmology tools and devices (tonometer, slit lamp, cameras)minus 80 freezer
- Spectrophotometer (not tested yet!)
- Surgivet anesthesia machine
- Western blot plates, mini-cells and Power Ease transfer voltmeter
- Isotemp water bath unit
Training or Skills
- DNA studies/extraction
- Microsatellite typing, and homozygosity mapping
- Perform DNA analyses using biochemical techniques, autoradiography and the PCV from blood and tissue samples
- Protein analysis with Western blotting, immunoprecipitation and staining procedures
- Gene analysis and therapy with novel vectors and protocols
- Cell culture creation and maintenance
- Create and edit high quality drawings and 2X2 slides
- Maintain and modify protocols for the evaluation of drugs influencing intraocular pressure.
- Collect, organize and analyze results and submit final data to faculty.
- Conduct independent statistical analyses using available software.
