Christopher D. Vulpe
- M.D., Medicine, University of California, San Francisco, 1996
- Ph.D., Biochemistry, Genetics, University of California, San Francisco, 1994
- S.B., Biology, Massachusetts Institute of Technology, 1986
Copper and Iron Metabolism
Copper and iron are vital nutrients with a highly conserved and interwoven metabolism that is required for the growth and development of all organisms. An overall research goal of the laboratory is to further understand copper and iron metabolism in mammals with a focus on 1) characterizing the role of the multi-copper ferroxidases (Fe (II)-> Fe(III) in iron homeostasis and 2) identifying the genetic factors that influence iron status in mammals using “in silico” QTL analysis of inbred mouse strains and collaborations to study genetic determinants of iron deficiency in zebrafish and humans.
Toxicogenomics and Green Chemistry
We are utilizing systematic functional analysis through the use of “barcoding” analysis in the budding yeast Saccharomyces cerevisiae to identify conserved toxicity pathways that may provide insight on toxicant susceptibility in people. We are currently focused on breast cancer carcinogens, mitochondrial toxicants, pesticides and emerging contaminants. Most recently we have started using whole genome CRISPR approaches in a similar approach in mammalian cell lines.
We are developing a novel approach for identifying and understanding the toxicity of xenobiotics in aquatic ecosystems by monitoring changes in global gene expression patterns in aquatic indicator species representative of different trophic levels, including Daphnia magna (a crustacean), and Pimephales promelas (fathead minnow). We are assessing the sensitivity, specificity and utility of an ecotoxicogenomics approach for ecological toxicity assessment in real world environmental settings. Tools we are using include traditional microarray technologies as well as high-throughput sequencing methods.
- DE Vidal-Dorsch, SM Bay, S Moore, B Layton, AC Mehinto, CD Vulpe. M Brown-Augustine, A Loguinov, H Poynton, N Garcia-Reyero, EJ Perkins, L Escalon, ND Denslow, CR Cristina, T Doan, S Shukradas, J Bruno, L Brown, GV Agglen, P Jackman, M Bauer Ecotoxicogenomics: Microarray interlaboratory comparability. Chemosphere 2016 144, 193-200.
- Pan S, Yuan C, Tagmount A, Rudel RA, Ackerman, JM, Yaswen, P, Vulpe CD, Leitman, DC. Parabens and Huan Epidermal Growth Factor Receptor Ligands Cross-Talk in Breast Cancer Cells. Environ Health Perspet. 2015 Oct 17.
- Vidal-Dorsch DE, Bay SM, Moore S, Layton B, Mehinto AC, Vulpe CD, Brown-Augustine, M, Loguinov A, Poynton, H, Gracia-Reyero, N, Perkins, EJ, Escalon, L, Denslow, ND, Cristina CR, Doan, T, Shukradas, S, Bruno, J, Brown, L, Van Agglen, G, Jackman, P, Bauer, M. Ecotoxicogenomics: Microarray Interlaboratory Comparability. Chemosphere 2015 Sep 9;144:293-300. doi: 10.1016/j.chemosphere.2015.08.019.
- Scanlan LD, Loguinov AV, Teng Q, Antczak P, Dailey KP, Nowinski DT, Kornbluh J, Lin XX, Lachenauer E, Arai A, Douglas NK, Falciani F, Stapleton HM, Vulpe CD. Gene Transcription, Metabolite and Lipid Profiling in Eco-Indicator Daphnia magna Indicate Diverse Mechanisms of Toxicity by Legacy and Emerging Flame-Retardants. Environ Sci Technol. 2015 Jun 16;49(12):7400-10. doi: 10.1021/acs.est.5b00977.
- Lee SM, Lee SB, Prywes R, Vulpe CD. Iron Deficiency Upregulates Egr1 Expression. Genes Nutr. 2015 Jul; 10(4):468. doi: 10.1007/s.12263-015-0468-0.
- Jiang R, Hua C, Wan Y, Jiang B, Hu H, Zheng J, Fuqua BK, Dunaief JL, Anderson GJ, David S, Vulpe CD, Chen H. Hephaestin and ceruloplasmin play distinct but interrelated roles in iron homeostasis in mouse brain. J Nutr. 2015 May;145(5):1003-9. doi: 10.3945/jn.114.207316.
- Gaytán BD, Vulpe CD. Functional toxicology: tools to advance the future of toxicity testing. Front Genet 2014 5:110; doi:10.3389/fgene.2014.00110.
- Fuqua BK, Lu Y, Darshan D, Frazer DM, Wilkins SJ, Wolkow N, Bell AG, Hsu J, Yu CC, Chen H, Dunaief JL, Anderson GJ, Vulpe CD. The multicopper ferroxidase hephaestin enhances intestinal iron absorption in mice. PLoS One. 2014 Jun 4;9(6):e98792. doi: 10.1371/journal.pone.0098792.
Additional Publications here