Regardless of the method of euthanasia used, death must be confirmed before disposal of the animal. The following should be used to evaluate consciousness or confirm death.
Lack of a heartbeat
Lack of respiration
Lack of a corneal reflex
The presence of a heart beat can best be determined with a stethoscope placed under the left elbow. Please note that a pulse is usually not palpable under such circumstances and should not be used to confirm death. Movement of the chest indicates respiration but respiration rates may be very erratic and slow in unconscious animals. Therefore, one must be cautious in the interpretation of respiration for confirmation of death. One may test for evidence of a corneal reflex by touching the surface of the eyeball. Normal or conscious animals will blink when the eyeball is touched. Absence of a corneal reflex, failure to detect respiration, and absence of a heart beat for a period of more than 5 minutes should be used to confirm death. An alternative is to observe the animal over a period of several hours. Lack of movement, absence of a heartbeat, respiration, or corneal reflex over an extended period of time provides further confirmation of death.
Special Considerations for Euthanasia of Bulls
Bulls present particular challenges because of size, attitude, and the physical thickness of their skull. Specialized heavy duty penetrating captive bolt guns or higher caliber firearms (9 mm or .357) are required for euthanasia of bulls. As described previously, safety is of paramount importance. Since ideal positioning of either device requires close contact with the animal, restraint is usually necessary. Operators should recognize that restraint alone causes significant distress. By preparing the euthanasia device for use prior to restraining the animal, one can limit the restraint-related stress period.
Unacceptable Methods of Euthanasia
The following is a partial list of methods considered as “unacceptable” for euthanasia of livestock:

• Manually applied blunt trauma to the head such as a large hammer

• Injection of any chemical substance not labeled for use as a     euthanasia agent

• Injection of air into a vein

• Electrocution as with a 120 or 220 volt electrical cord


Under Florida law use of any of the above methods for euthanasia of livestock may be considered a violation of Chapter 828.12 which states that: A person who intentionally commits an act to any animal which results in the cruel death, or excessive or repeated infliction of unnecessary pain or suffering, or causes the same to be done, is guilty of a felony of the third degree, punishable as provided in section 775.082 or by a fine of not more than $10,000 or both.
Personnel Training
Large farms and ranches are advised to develop personnel training programs for proper instruction of humane euthanasia techniques. As indicated in the previous discussion, the skill and experience of personnel are of paramount importance when gunshot or penetrating captive bolt/exsanguination are used for euthanasia of sick and debilitated animals. Experience has shown that many people (even those experienced in handling livestock) are not aware of the anatomical landmarks for proper execution of these techniques. Furthermore, persons should be aware that there is significant danger for the operator (or for bystanders with gunshot) whenever these methods of euthanasia are used. On large farms or ranches, most, if not all, persons should be familiar with these procedures and several should be specifically trained to perform this task. However, only those who can demonstrate a working knowledge and proficiency with the techniques should be permitted to perform euthanasia procedures. When these methods are not properly performed, animals may become injured, have varying degrees of consciousness, and experience needless pain and distress.

Experienced persons should assist in the training of inexperienced persons and utilize carcasses to demonstrate anatomical landmarks and application of the various techniques. Carcasses should be used for practice by trainees until they become competent with the procedures. People must also be aware of how to confirm death. In some cases this may require specific training with, and observation of, live animals.
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