College of Veterinary Medicine

Education:

M.S., Biochemistry, Yale University
Ph.D., Molecular Biology, University Of Florida

Honors and Awards:

 

Research Interests:

Her research interests include discovering biomarkers at both the mRNA and protein levels that relate to exposure of fish to environmental contaminants. In particular she is interested in defining molecular mechanisms of action of endocrine disrupting compounds that adversely affect reproduction. Her research covers both sex hormone receptor mediated and independent mechanisms. Favorite model systems include largemouth bass, fathead minnow, sheepshead minnow and zebrafish. Common research tools include microarrays, real time PCR, proteomics, tissue culture based assays, transfections and in vivo determination of reproductive endpoints.
In addition, Dr. Denslow has initiated research to understand the effect of nanomaterials on fish health. For this research, zebrafish microarray analysis is used as a tool to suggest global pathways that may be affected by exposure. These experiments are integrated with others looking at gill function, histopathology, nanomaterial uptake and nanomaterial characterization.

Selected Publications:

For Publications Extracted From Medline Click HERE

Ottens, A.K., F. Kobeissy, E.C. Golden, Z. Zhang, W.E. Haskins, S-S. Chen, R.L. Hayes, KKW. Wang, and N.D. Denslow, Neuroproteomics in Neurotrauma. Mass Spect Review, 25: 380-408 (2006).

Eidem J.K., H. Kleivdal, K. Kroll, N. Denslow, R. van Aerle, C. Tyler, G. Panter, T. Hutchinson, A. Goksoyr. "Development and validation of a direct homologous quantitative sandwich ELISA for fathead minnow (Pimephales promelas) vitellogenin." Aquat Toxicol. 78:202-206 (2006).

Kobeissy F.H., A.K. Ottens, Z. Zhang, M.C. Liu, N.D. Denslow, J.R. Dave, F.C. Tortella, R.L. Hayes, K.K. Wang."Novel differential neuroproteomics analysis of traumatic brain injury in rats."Mol Cell Proteomics. 5: 1887-1898 (2006).

Garcia-Reyero, N., D.S. Barber, T.S. Gross, K.G. Johnson, M.S. Sepulveda, N.J. Szabo, N.D. Denslow. "Dietary exposure of largemouth bass to OCPs changes expression of genes important for reproduction." Aquat Toxicol. 78:358-369 (2006).

Knoebl I, Blum JL, Hemmer MJ, Denslow ND.Temporal gene induction patterns in sheepshead minnows exposed to 17beta-estradiol. J Exp Zoolog A Comp Exp Biol. 305:707-719 (2006).

Garcia-Reyero, N., and Denslow, N.D.Applications of genomic technologies to the study or organochlorine pesticide-induced reproductive toxicity in fish.J. Pestic. Sci 31: 252-262 (2006).

Barber DS, McNally AJ, Garcia-Reyero N, Denslow ND.Exposure to p,p'-DDE or dieldrin during the reproductive season alters hepatic CYP expression in largemouth bass (Micropterus salmoides). Aquat Toxicol. 81:27-35 (2007).

Villeneuve DL, Larkin P, Knoebl I, Miracle AL, Kahl MD, Jensen KM, Makynen EA, Durhan EJ, Carter BJ, Denslow ND, Ankley GT.A graphical systems model to facilitate hypothesis-driven ecotoxicogenomics research on the teleost brain-pituitary-gonadal axis. Environ Sci Technol. 41:321-30 (2007).

Denslow, ND, Garcia-Reyero, N., and Barber, D.S.Fish ‘N’ chips: the use of microarrays for aquatic toxicology. Mol. BioSyst.,3: 172-177 (2007).

Larkin, P., Villeneuve, D.L., Knoebl, I., Miracle, A.L, Carter, B.J., Liu, L., Denslow, N.D. and Ankley, G.T.Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas).Environ. Toxico. and Chem. 26: 1497-1506 (2007).